分子内的

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

Our interpretation of the results from parsimony, distance and maximum likelihood based phylogenetic analyses suggest:(1) African colobines and Asian colobines have been independent evolutionary entities for a long time; the divergent time is estimated to occur 10 million years ago based on a molecular clock of 2% substitution per site per million years;(2) both Rhinopithecus and Trachypithecus could be regarded as separate genus;(3) Pygathrix and Rhinopithecus are sister groups while Presbytis and Trachypithecus are closely related;(4) both the Sichuan and Yunnan snub-nosed monkey represent valid species respectively;(5) within genus Trachypithecus, T. phayrei is closely related to T. cristatus and T. auratus, while T. francoisi is distinct from them; the white-headed leaf monkey, however, should be a subspecies of T. francoisi;(6) within genus Presbytis, P. thomasi should be separated as a valid species from P. melalophos, but the genetically divergent degree between P. comata and P. melalophos implies that P. comata may just be a subspecies of P. melalophos.

结果表明：（1）非洲疣猴与亚洲疣猴的分歧已非常明显，按分子钟推算的分歧时间约在1，000万年前；（2）Rhinopithecus和Trachypithecus均应成为独立的属；（3）白臀叶猴属与金丝猴属关系较近，Presbytis属和Trachypithecus属关系也较近；（4）我国的川、滇金丝猴应分别被视为独立的物种；（5）在Trachypithecus属中，菲氏叶猴与银叶猴(T.cristatus)和乌木叶猴亲缘关系较近，黑叶猴(T.francoisi)则与它们的关系较远，曾被怀疑为一个种的白头叶猴(T.f.leucocephalus)应只是黑叶猴的一个亚种；（6）在Presbytis属内，托氏叶猴应从僧帽叶猴(P.melalophos)中独立出来成为一个有效种，但我们的结果倾向于将灰发叶猴置为僧帽叶猴的一个亚种。

This study built up an experimental rats model to imitate human stercotypm of movement, it provide the result on the biochemical changes of the brain in schizophreniac.

同时，通过本课题周年节律的研究，为《内经》&时藏阴阳&理论找到了现代科学的内涵，在现代科技高速发展的今天，在西医学从细胞、分子水平观察和研究生命现象的背景下，开辟新的研究模式和思路，抓住某一个或几个单一节律的变化规律，从宏观、动态的角度加以认识，为中医的现代化研究及中西医结合找到共同语言和突破口，也是本课题研究的目的之一。

The instrument may be used in measuring sheat wave resonant absorption spectrum of liquid film in 1x10 to the power -5 -500 Hz frequency range and in providing measurement data for the research of the interaction range between clusters, long chains and multibands of liquid film, the group behavior of molecules and clusters, the long chain in polymer solution, the second, the third and the fourth group behavior in left liquid and other group behavior in chemical solution and complex.

本发明的谱仪可在10 －5 ―500Hz频率范围内，测量液体膜的切变波共振吸收谱，为研究溶液中的团簇间、长链间和多键间的交互作用范围，分子和团簇的集团行为，高分子溶液中的长链，生命液体中的二、三、四级或其他化学溶液和络合物中的基团的行为提供可靠的测量。

Internal dose ones and external dose ones are concluded.(2) Biomarker of effect. It indicates those indicaters inflecting the biochemical, biophysical, behavioral, or else changes. Three types of biomarkers, early biological effect, altered structure / functon and disease, are comprised.

生物标志物是包括反映生物系统与环境中化学、物理或生物因素之间相互作用的任何测定指标，可将其分为三类：(1)接触的生物标志物，即反映机体生物材料中外源性物质或其代谢物或外源性物质与某些靶细胞或靶分子相互作用产物的含量，包括反映内剂量和生物效应剂量两类标志物；(2)效应的生物标志物，指机体中可测出的生化、生理、行为或其他改变的指标，包括反映早期生物效应、结构和功能改变(altered structure/functon)及疾病3类标志物；(3)易感性的生物标志物，即反映机体先天具有或后天获得的对接触外源性物质产生反应能力的指标。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

Particularly well-oriented mesochannels in mesostructured transparent thin films on solid substrates are of great interest because the functinalization of mesochannels in mesostructures has allowed one to confine organic functions in nanostructures,which have potential applications for hard coatings,energy conversion,solar cells,molecular separation,pH sensors by incorporating pH-sensitive dyes,dielectric layers,and sensing of chemical and biological analytes.

在固体基质上的介观结构透明薄膜中取向非常好的介观通道拥有巨大的研究价值，因为介观结构中的介观通道的功能化石我们能够把有机官能团限制在纳米结构内，这样就在硬质涂层、能量转换、太阳能电池、分子分离、由pH敏感的染料体现的pH传感器、绝缘层和化学和生物分析物的传感方面都有潜在的应用。

Guanine can quench the phthalhydrazidylazoacetylacetone's ECL in acid medium, and the linear concentration range of guanine relative to the ECL intensity is from 2.0×10~(-6) to 1.0×10~(-5) mol/L.The mechanism of phthalhydrazidylazoacetylacetone's ECL on the surface of ITO glass has been discussed according the experimental phenomenon.The phenomenon of ITO self-luminescence has also been studied.It has been found that the machanism of phthalhydrazidylazoacetylacetone on the surface of ITO in different medium is dissimilarity. The phenomenon of ITO self-luminescence may be caused by the interaction of the active oxygen element and ITO's surface.

鸟嘌呤在弱酸性介质中3-(1-乙酰丙酮偶氮)苯二甲酰肼的电化学发光具有显著的淬灭作用，2.0×10~(-6)～1.0×10~(-5) mol/L浓度范围内，电化学发光强度与鸟嘌呤的浓度成良好的线性关系根据相关试验现象对3-(1-乙酰丙酮偶氮)苯二甲酰肼在氧化铟锡玻璃电极上的电化学发光机理进行了初步探讨，并对氧化铟锡玻璃电极自身发光现象进行了研究，研究发现在不同酸度环境中，3-(1-乙酰丙酮偶氮)苯二甲酰肼在氧化铟锡玻璃电极上电化学发光的机理是显著不同的；ITO自身发光可能是由活性氧系分子与ITO镀膜玻璃电极表面相作用引起的。

Researchers expected that the gene responsible would vary from lake to lake. Instead, they found that each group of stranded sticklebacks had lost its armor by the same mechanism: a rare DNA defect affecting a signaling molecule involved in the development of dermal bones and teeth. That single preexisting variant--rare in the open ocean--allowed the fish to adapt rapidly to a new environment.

研究者希望不同的湖之间相关的基因有所差别，可是，他们发现每一组搁浅的棘鱼都通过同样的机制失去了它们的装甲：一种罕见的DNA缺陷影响了一个涉及皮内骨和牙齿生长的信号分子，这一个先前存在的变数——在开放的海洋中很少存在——使得鱼可以迅速适应新的环境。

He has memorably depicted the slimy and seemingly-traitorous Severus Snape in the 'Harry Potter' films, the greedy and perverted Judge Turpin in 'Sweeney Todd' and terrorist Hans Gruber in the original 'Die Hard.

他以令人过目难忘的演技刻画了HP中油腻腻的看上去叛逆的斯内普，《理发师陶德》中贪婪邪恶的法官Turpin以及《虎胆龙威》中的恐怖分子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。