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To observe the effect of serum containing Carapax et Plastrum Testudinis on proliferation of rat mesenchymal stem cells.

观察益肾中药龟板血清对大鼠骨髓间质干细胞(mesenchymal stem cells，MSC)体外增殖的影响。

Results Plastrum Testudinis can decrease the neurologic dificit score and promote the expression of Nestin...

提示补肾中药龟板可减轻神经损伤症状和对局灶性脑缺血后神经干细胞有促进增殖作用。

Promoting effect of Seropharmacological Plastrum Testudinis on proliferation of MSC might be relate to the up-regulated expression of PCNA.

龟板血清促MSC增殖作用可能与上调PCNA表达有关。

To observe the effect of serum containing Carapax et Plastrum Testudinis in inducing the differentiation of mesenchymal stem cells of adult rats into neurons.

观察龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元的能力。

Objective To observe the effect of Seropharmacological Plastrum Testudinis on Proliferation cell unclear antigen of rat mesenchymal stem cell in vitro.

目的：观察益肾龟板血清对大鼠骨髓间质干细胞(mesenchymal stem cells， MSC)体外增殖过程的增殖细胞核抗原(Proliferation cell nuclear antigen， PCNA)表达的影响。

The growth of rat mesenchymal stem cells with low,middle and high seropharmacological plastrum testudinis were observed separately by means of morphological apperances.

结果BMP4蛋白和mRNA表达相一致，龟板含药血清以浓度依赖方式促进MSC的BMP4 mRNA及其蛋白表达。

Results: Seropharmacological Plastrum Testudinis can promote the number of PCNA positive cell and the expression of PCNA protein in dose-depend-ant, there axe significant difference in comparison with control groups.

结果：龟板血清以浓度依赖方式促进MSC的PC-NA及其mRNA表达阳性细胞数和PCNA蛋白表达，与对照组比较有显著性(P.05)。

Methods The activitie of anti-lipidperoxidation of Chinemys Plastrum Testudinis of petroleum-ether, acetoacetate and 95% alcohol solution from two producing areas was studied by DPPH.

方法采用DPPH法分别测定两种不同产地龟板的石油醚、乙酸乙酯、95％乙醇等不同极性溶剂提取物的抗氧化活性。

Plastrum Testudinis ; Mesenchymal stem cells ; Osteoblast ; Pharmacology

龟板；骨髓间充质干细胞；成骨细胞；药理学

CARAPAX ET PLASTRUM TESTUDINIS/pharmacology ; REINFORCING KIDNEY ; MYELOID PROGENITOR CELLS ; CELL CULTURE ; IMMUNOHISTOCHEMISTRY

龟板/药理学；补肾；骨髓祖代细胞；细胞培养；免疫组织化学

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。