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龙血树

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Through the analysis of the contents of loureirins of the zymolysis products of fungus with different culture time, it can conclude that dragon's blood is the products of interactions between Dracaena cochinchinenis and the animalcula.

对比部分微生物不同培养时间的发酵产物,可以得出龙血竭的形成是剑叶龙血树和它损伤后感染的微生物相互作用的结果,并且从微生物回接实验的结果来看,某些微生物对剑叶龙血树龙血竭的形成有一定的促进作用。

In chapter 3, the animalcula of Dracaena cochinchinenis's wound were carefully isolated, it also argues the effect of fungi on the formation of the dragon's blood. Moreover, the loureirins of the liquid zymolysis products of some fungi were detected by HPLC, and the relationship between animalcula and the formation of the dragon's blood is further discussed.

第三章详细地论述了剑叶龙血树微生物群的分离、纯化及其对龙血竭形成的影响,并用HPLC对部分微生物的液体发酵产物作龙血素成分的检测,更进一步地了解微生物与剑叶龙血树龙血竭形成的关系。

Objective:To study the anatomy of Dracaena cochinchinensis systematically,and find out the distribution and detect the constituents of its resin,in order to provide substantial foundation for the formation mechanism of its red resin.

中文摘要:目的:对剑叶龙血树进行系统的组织学研究及所含树脂的分布和成分检测研究,为进一步研究剑叶龙血树树脂形成的成因和机理提供参考依据。

There are six kinds of Dracaena in China. Dracaena cambodiana Pierre ex Gagnep is peculiar in Hainan province.

龙血树属植物我国产6种,其中海南龙血树(Dracaena cambodiana Pierre ex Gagnep)又名小花龙血树,是海南的特有树种。

In chapter 2, the contents of loureirins in the roots, stems and leaves of Dracaena cochinchinenis were analyzed by HPLC. On the other hand, using the preparation chromatography technology, five compounds were isolated from the leaves of Dracaena cochinchinenis, their structures were identified by means of spectral and chemical methods. They are steroidal saponins.

第二章主要是利用HPLC技术对剑叶龙血树根、茎、叶中龙血素成分的分析与探讨,并利用制备色谱等技术对剑叶龙血树叶子组成成分进行了分析、研究,得到化合物5个,经波谱鉴定,这5个化合物均是幽体皂贰类化合物。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料,通过对龙血树幼嫩茎段两步培养法再生的研究,建立了龙血树幼嫩茎段离体再生体系,证明了愈伤组织的发生和激素种类与浓度的关系;并在此基础上研究了愈伤组织再生对不同人工诱变方法(60Co辐射、EMS、秋水仙素)的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性,得到了2株变异株系。

The analysis of the chemical constitutes among sterns roots and leaves of Dracaena cochinchinenis, showed that there' s small amount loureirin A in their extracts, with 0.053mg/g, 0.058mg/g, 0.015mg/g respectively only. Therefore, the roots, stems and leaves of Dracaena cochinchinenis have too low contents of loureirins to be raw materials of dragon's blood.

分析剑叶龙血树根、茎、叶的成分,发现风干的剑叶龙血树根、茎、叶中没有龙血素B的存在,只含有少量的龙血素A,其含量在根、茎、叶中分别为:0.058mg/g,0.053mg/g,0.015mg/g,其含量远低于现行的龙血竭中龙血素A含量不低于2%的质检标准,因此剑叶龙血树的根、茎、叶不能直接用作生产龙血竭的原料。

Its isolation has created separate flora and fauna from the mainland, including the Dragon Blood Tree, so named because it leaks a bright red sap, said to be the blood of dragons.

索科特拉岛位於印度洋上,离叶门本土五百公里处,它孤立隔绝的地理位置孕育出有别於大陆的动植物,包括会流出鲜红树汁的龙血树,这种树汁据称是龙血。

Result:Characteristics of the tangentical longitudinal section of stem with resin and surface view of leaves were elucidated.Besides xylem vessels and fibers of the stem,it was found that the red resin also exists in the cortex parenchyma cells of the stem and the medulla and xylem of the root.According to the HPLC fingerprint analysis result of the stems with and without resin,a number of flavones and stilbenoids were detected in the stem in which resin appeared after it wounded.

结果:阐明了含树脂茎的纵切结构以及叶的表面观特征;除了茎木质部导管和纤维中有树脂外,发现剑叶龙血树受到损伤之后在茎皮层薄壁细胞、根的髓部和木质部亦有树脂的分布;含树脂茎和无树脂茎的指纹图谱显示剑叶龙血树茎在形成树脂后的化学成分中出现了较多的黄酮类、茋类成分。

In chapter 4, for the further study on dragon's blood, the ecological mechanism of the formation of dragon's blood was tentatively analyzed.

第四章在前人的研究成果基础上结合本实验结果,初步分析了真菌与剑叶龙血树龙血竭形成的相互作用关系,试图为进一步解释龙血竭的形成提出可能的实验依据。

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