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MATERIALS: Femur and tibia of F344 male rats aged 2 months were sterilely harvested to prepare BMSCs.

材料:无菌条件下取2月F344雄性大鼠股骨、胫骨制备骨髓间充质干细胞。

Five male Wistar rats of 4-5 weeks old were executed by cutting off the heads to sterilely obtain the muscle group in hind limbs, remove the fat and the fascia, so as to prepare for single cell suspension.

选取4~5周Wistar雄性大鼠5只,脱颈处死无菌切取后腿肌群,剪除脂肪和筋膜,制备单细胞悬液。

METHODS: Bilateral testis of Kunming male mice aged 5-7 days were sterilely collected. Spermatogonial stem cells were isolated and purified using adherence and noncontinuity Percoll density gradient centrifugation.

无菌收集5~7 d昆明雄性小鼠双侧睾丸,采用差速贴壁联合非连续性Percoll密度梯度离心法分离纯化精原干细胞。

Method]Based on perineal striae,root-knot nematodes of greenhouse's vegetable in Luoyang were identified and toxicities of four kinds of fungicides to the root-knot nematodes were tested.

方法]采用传统的制作会阴花纹的方法,对洛阳温室蔬菜根结线虫的种类进行鉴定,并选择4种药剂对根结线虫的二幼虫进行室内毒力测定。

Three hundred one-day-old avian broiler whose body weights were near consistent and healthy were selected, and were divided into 5 disposes randomly, 3 repeats in each dispose. Adopting single factor to compare the effect of complex amino acid trace element chelated on production performance, death rate and disease occur rate in leg of broiler when it was added with high Cu (Cu 250 mg/kg) level, 1 times NRC level, 0. 5 times NRC level, 0 level (neither use chelated nor sulphated) and 1 times NRC level as sulphate.

选体重近似一致、健康的1日艾维茵肉用仔鸡300只,随机分为5个处理,每个处理3个重复,采用单因子设计比较研究了复合氨基酸螯合微量元素(Fe、Cu、Mn、Zn)高铜水平(Cu 250 mg/kg)、NRC水平、0.5倍NRC水平、0水平和硫酸盐对肉用仔鸡生产性能、腿病发生率和死亡率的影响。

The experiment, in which supraoesophageal ganglions of the fifth-instar larvae were removed, proved that SG plays an important role in the formation of diapause eggs.

通过对家蚕末幼虫咽下神经节的摘除试验证实,咽下神经节对滞育卵形成起着重要作用。

METHODS: ADSCs were prepared by collagenase I digestion of subcutaneous fat from the suprascapular site of Japanese white rabbit.

1.2.1 兔ADSCs(rabbit ADSCs, rADSCs)的原代培养 3 mo成年日本大耳白兔,耳缘静脉注射空气处死。

MATERIALS: The experiment was conducted in the Experimental Animal Center and the Anatomy Department of Sun Yat-sen University from April 2003 to February 2004. Thirty-two APPSWE transgenic mice of 5 months old were bought from Taconic Company, USA.

材料:实验于2003-04/2004-02在中山大学实验动物中心和人体解剖学教研室脑研究室进行。5月APPSWE转基因小鼠32只,购自美国Taconic company,在本室繁育成功。

The enzyme activity of cotton bollworm was indicated; after 144 hours teated with the subthal dose (LC50) of the pesticiedes, the difference was detected in the GSTs activities when compared with contrast.

酶动力学研究表明:用4种药剂的亚致死浓度LC50剂量处理棉铃虫3幼虫,在药剂处理后的144h内,幼虫GSTs活性发育变化规律与对照有一定的差异。

The depth of tectal layerⅠand the morphological characteristics of tectal cells in layerⅠwas investigated using Nissle staining, Golgi staining, and postmortem labeled with DiImethod. Most ofⅠcells appeared shuttle, others showed round, oval, pyramidal, triangular,and polygonal etc. The lateral region of layerⅠwas thicker than the ventral and the dorsalregions.

本研究使用Golgi-Cox法,灌流固定后尼氏体染色、羰花青荧光染料DiI(1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbo-cyanine perchlorate)逆向神经标记技术,对雏鸡视顶盖Ⅰ层的厚度和该层的细胞形态、大小进行了观察研究和数理统计,并对不同日鸡胚的Ⅰ层厚度进行了数理统计及分析研究。

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