龄

The highest transformation frequency 11.21% was achieved on the optimization facts, which were medium YEB with pH5.6, bacterial concentration OD 600=0.4, precultivation for one day, cocultivation for four days, the coculaivation media supplemented with GA3 0.5 mg/L and leaf explanst growed for 45 days.

研究了影响转化频率的主要因素，得出在使用pH5.6的YEB培养基，菌液浓度OD600＝0.4，45日苗龄中部叶片预培养1 d，共培养4 d，共培养的培养基中加入0.5 mg/L GA3的条件下可使转化频率提高到11.21%。

Results showed that the frequencies of shoot regeneration of cotyledons explants from 4 d old in vitro grown seedlings were markedly increased from 34% to 46% via a preculture procedure of 2 days period using the media containing the hormone combinations of 2,4 D 0.5-1.2 g/L and 6 BA 0.2 mg/L.

结果表明，苗龄4d的幼苗子叶于含2，4－D0.5－1.2mg／L和6－BA0.2mg／L的培养基培养2或6d后再转到分化培养基上培养，芽再生率提高34％－46％。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

For L. barbarum transformation, the optimized procedure was using 3-week-old leaf explants, preculture for 3-4 days, infection with 1∶1 dilution of an overnight culture of EHA101 (pIG121Hm) suspension with AS and co-culture for 3 days at 24℃ in the dark.

通过农杆菌转化条件的优化，将AS活化的过夜培养EHA101（pIG121Hm）菌液稀释一倍后感染预培养3—4天的三周龄叶外植体，并在24℃黑暗条件下共培养3天，可以获得较为理想的转化效率（62.5％）。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

The data of RT-PCR also indicated that, in prepubertal rat (20-day-old) tissues, GLUT8 expression was comparatively much lower than that in the adult rat tissues.

RT-PCR数据还显示，在20d龄的青春前期大鼠的不成熟睾丸和心、肝、肾、脾等其它组织器官中，GLUT8基因表达水平相对于成年大鼠要低一些。

A monolayer culture system of pituitary cells was used to study the release of LH and FSH and their responses to a GnRH agonist,LRH-A3,in vitro by pituitary cells of prepubertal gilts at the ages of 30,60,90,120,and 150 days.

利用垂体细胞单层培养模型研究了30，60，90，120和150日龄北京黑猪母猪垂体细胞LH和FSH的释放及其对LRH-A3反应能力。

Restoring natural areas to presettlement conditions should not be done if it means utting down mature trees.

如果恢复自然区域会砍伐熟龄树木就不可行

Methods］ 36 twomonthold rabbits with white feather and black eyes,half male and half female,were randomly divided into 6 groups:normal group,model group,xiangsha liujun pill group, lizhu changle group, lowdose leweiyintreated group,highdose leweiyintreated group.except the normal group, others were made into ibs model of spleen deficiency which was induced by damp heat stress and gavage with fanxieye as well as method of either hunger or gorge.the drug groups were administered preventively from the mediumterm of the model reproduction to the end of modeling.the rabbits of each group were observed.no,nos,mda,sod in serum and sp in colonic membrane were detected.

方法］两月龄白毛黑眼兔36只，雌雄各半，随机分为6组：正常组、模型组、香砂6君丸组、丽珠肠乐组、乐胃饮低剂量组、乐胃饮高剂量组。除正常组外，其余whbe兔采用湿热环境应激联合番泻叶灌胃加饥饱失常复合方法致脾虚泄泻型ibs模型。从模型复制的中期开始各用药组预防给药，至造模结束。观察各组1般情况，测定血清no、nos、mda、sod含量及结肠黏膜组织中sp含量。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3，并对它们的部分生物学特性进行研究。将病毒通过9-11日龄鸡胚，盲传5-6代。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。