龄

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

Over the past few days and watch Heling words: Luandan inside the long hair; chicken can be several feet out of 3; Yingdu on the existence of the world; dogs can be named as sheep, horse can be said to be the oviparous; can Xiama There are said to be a long tail; the fire itself does not sense heat; in the mountains echo the mountains also give birth to prove the mouth; wheels will never be a way; eyes to see as a lack of substantial capacity; to identify foreign objects can not always be the real thing; turtle May be longer than a snake; Square can not draw a square and compasses can not be used to Circle; own mortise and Suntou not completely fit; birds can be seen there had been no movement; the arrow flies off a Stay, there are not stop time; puppy dogs do not have to; Ma Huang, the Black Cattle can name three reproach; dog might as well call it the black dog; known as the lone horse that it should not have had the mother; foot Long sticks, the interception of 50 per day, 10,000 cut-off points are a never-ending, an endlessly Landiao.

而几天来观看鹤龄的言辞：卵蛋里面长着毛；鸡的脚可以数出三只；郢都内就存在着天下；狗也可命名为羊，马能够说是卵生的；虾蟆可以说是长有尾巴；火本身并没有热感；山中的回音证明大山也生出了口；车轮永远不会着地；眼睛缺乏看视实质的能力；指认外物永远达不到事物的实际；乌龟可能比蛇还长；角尺不能画出方形，圆规也不能用来画圆；自己的榫眼与榫头不会完全地吻合；飞鸟的身影也可说不曾有过移动；飞逝而去的箭头有停留、也有不曾停歇的时刻；小狗可以不是狗；黄马、黑牛的称谓可以数落出三个；白狗也可以叫它黑狗；称作孤驹应该说它不曾有过母亲；一尺长的棍棒，每天截取一半，一万年也分截不完，一篇滥调没完没了。

Wu Baiyan,Xian Meiwei,Shang Kegang,Wu Heling

吴白燕1，冼美薇2，尚克刚2，吴鹤龄2

Heliothis armigera is a kind of very important pest in agriculture,and causes great economic loss in our country and other countries every year.

棉龄虫是我国一种重要的农作物害虫，给我国和世界各国带来了巨大的经济损失。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料，通过9～11 日龄非免疫鸡胚进行了病毒的实验室分离和传代，并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定，结果表明，4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞，标准阳性血清可特异性的抑制其凝集性，回归试验可复制出与自然发病相同的病例，病毒传代物有明显的致鸡胚矮小化作用，透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子，将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

Hg4-03 on intestinal microflora of the 4th instar Hepialus gonggaensis larvae, an economically important insect.

Hg4-03作为食物添加剂对实验室饲养的4龄贡嘎蝠蛾幼虫肠道菌群的影响。

The company since had been established continues each kind of exposition, exhibition hall, the exclusive agency, The conference scene manufacture and booth building, accumulated the rich scene experience, Our customer proliferates anywhere, once has served the brand has HERBORIST , Bio-essence , Ginvera , Tokalon , Totole , Triumph , Bailing Medcine and so on.

公司自成立以来承接各类展览会、展厅展柜、专卖店，会议现场的制作与展台的搭建，积累了丰富的现场经验，我们的客户遍布大江南北，曾服务过的品牌有佰草集、凯伊秀、诗丽雅、妥佳侬、太太乐、黛安芬、百龄药业等。

And Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp.

该菌株的发酵液对大豆胞囊线虫二龄幼虫具有较高的毒杀活性并显著抑制胞囊孵化，不同稀释浓度发酵液的处理和无菌水处理有显著差异，发酵原液的杀线虫效果可达到90％以上，温室防效试验效果明显。同时该菌株对根结线虫Meloidogyne spp。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Meanwhile, in the CA3 area of hippocampus in NS group, a small amount of N-Cadherin positive cells were visible and were slightly stained. Compared with NS group, in PTZ seizure group, N-Cadherin positive cells had a marked increase in area CA3 and dentate hilus (P.01), but they were strongly inhibited in PDTC group (P.05). There was no correlation between the expressions of N-cadherin, Timm-stained granules and between the age of rats with seizure.

同时，NS组14天和28天大鼠海马CA3区可见少量的N-Cadherin阳性细胞，着色不深；PTZ致惊组海马CA3和齿状回门区的N-Cadherin阳性细胞与NS组相比明显增多（P＜0.01），PDTC预处理后相同区域内N-Cadherin阳性细胞较PTZ致惊组明显减少（P＜0.05）。N-Cadherin与Timm染色颗粒的表达结果与惊厥鼠的日龄并无关联性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。