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Keywords: Trillium tschonoskii; ISSR; AFLP; genetic diversity; in vitro culture

中文关键词:延草;ISSR;AFLP;遗传多样性;离体培养

Trillium tschonoshii Maxim, a species belonging to the family Liliaceae, was listed as national protect object (Grade 3), which is also of important medicine values.

草为百合科植物,属国家Ⅲ级重点保护的野生植物,具有重要的药用价值。

Any liliaceous plant of the genus Trillium having a whorl of three leaves at the top of the stem with a single three-petalled flower.

草属的多种百合科植物之一,三片叶在茎顶端轮生,花单生、有三片花瓣。

Trillium seeds have a fleshy organ called an elaiosome that attracts ants.

草种子有肉质器官称为 elaiosome,吸引蚂蚁。

Genetic diversity among 7 populations of Trillium tschonoskii were analyzed by using inter SSR and amplified fragment length polymorphism molecular markers. The results were as follows:(1)ISSR: 12 out of the 100 primers showed consistent banding patterns and amplification, a total of 135 bands were produced with an arrange of 250-2500bp, 46 of which (34.07%) were polymorphic.

应用锚定SSR和限制性片段多态对7个延草居群的遗传多样性进行了分析,结果如下:从100个引物中筛选出12个ISSR引物,在250-2500bp之间共扩增出的135个条带,其中多态性带为46,占34.07%。

In our close collaboration with psychologist Susanne Cook-Greuter—and our 25 years of extensive survey-based consulting at companies such as Deutsche Bank, Harvard Pilgrim Health Care, Hewlett-Packard, NSA, Trillium Asset Management, Aviva, and Volvo—we've worked with thousands of executives as they've tried to develop their leadership skills.

在心理学者苏珊·库克·格罗伊特的密切协作下,我们25年里进行了广泛的以调查为基础的咨询研究。我们调查的企业、机构包括德国的德意志银行,美国的Harvard Pilgrim Health Care 基金会,惠普,美国安全局,美国的延草资产管理公司,美国的Aviva通讯公司,和瑞典的沃尔沃集团公司等。我们与这些公司和机构的数千位执行官或经理们共同工作并发展他们的领导艺术。

To 7 days' larve alter hatching larvae mostly lived on copepod nauplii, copepodites and trochophore of Polychaeta in water of the earthen ponds, apart from feeding on particulate organic detritus.

孵化后5~7日仔鱼基本上依靠摄食土池水体中的桡足类无节幼体、桡足幼体和多毛类担轮幼体,但还摄食少量的颗粒有机碎屑。

Methods PMSG-HCG were injected into 5 weeks SD rats. Embryos were collected by tearing intumescences of oviduct or clysising fimbriae tubae. The amount of embryos, rates of fertilization, and survival rate of embryos after injection were analyzed to compare the two methods.

选用最佳超排5周SD大鼠,腹腔注射PMSG-HCG,分别用撕开输卵管壶腹部和输卵管伞端冲洗两种方法采集胚胎,比较获得卵数,受精率,注射存活率等的差异。

Part three:Cochlear on Hair Cell Like Cells Regeneration in Presbycusis C57BL/6 Mice after Math1 Gene Inner Ear TransfectionIn order to test the probability of Mathl gene induces hair cell like cells regeneration in cochlea of presbycusis C57BL/6 mice,8 mounths old C57BL/6 mice were used for this study and were microinjected with Ad-MathlorAd-EGFP in the scala tympani by ventral approach.

第三部分老年性聋模型小鼠C57BL/6耳蜗鼓阶外源基因Math1导入后出现毛细胞样细胞现象的观察为了观察老年性聋C57BL/6小鼠(8月)耳蜗鼓阶外源基因Math1导入后能否在老年性聋的耳蜗微环境诱导出新生的毛细胞样细胞,为Math1基因治疗老年性聋进行探索性研究。

The results demonstrated that NHE1 transcripts were expressed ubiquitously.

结果显示, NHE1 mRNA在两种胎的多种组织中均有表达。

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