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Allantois and surrounding tissues of 8.5 day post coitum embryos, the hindhut and surrounding tissues of 9.5 dpc and 10.5 dpc embryos, the gonadal ridges and surrounding tissues of 11.5 dpc and 12.5 dpc embryos were collected and digested with 0.25%pancreatin-0.02%EDTA, then the cells were cultured in the plastic petridishes which are pretreated with 0.1% gelatine.

实验方法:取胚8.5 d胎鼠尾端尿囊及周围组织,取胚9.5~10.5 d胎鼠后肠及周围组织,取胚11.5 d,12.5 d胎鼠生殖嵴及周围组织,分别经0.25%胰酶-0.02%EDTA消化并接种于0.1%明胶包被的培养皿中。

Group I at 21 and 25 weeks of age and Group Ⅱ only at 25 weeks of age were vaccinated subcutaneously in the nape of the neck with 0 5 ml of MG oil emulsion bacterin respectively.

本试验将30只MG阴性的20周的蛋种鸡随机分为4组,Ⅰ组于21和25周、Ⅱ组仅于25周时分别颈部皮下接种MG油乳剂灭活苗0.5 ml/只,Ⅲ组于攻毒后1周接种1 ml/只,另设一组对照,不接种疫苗。

Of our native wild flowers, and the same bad bloodcrops out in the purple trillium or birthroot.

在我们故乡最漂亮的野花当中,它是延草的第一个近亲,并且,在紫色的延草或直立延草中,同样令人生厌地裸露在地面各处。

The mean weight of genetype AB(19.73kg) was the highest and the mean body length of genetype DD(56.00cm ) was the longest in microsatellite IDVGA30 in 90 days old. In microsatellite BMS1516, the chest high of genetype EF(22.44cm), the heart girth (62.28cm)and the circumference of cannon bone (8.07cm) of genetype CD were the highest in Chinese Merino lamb of 90 days old. In microsatellite OY3, the born weight of genetype EF was the highest(4.10kg).

其中微卫星座位IDVGA30 AB基因型90日平均体重最大(19.73kg),DD基因型90日平均体长最大(56.00cm);微卫星座位BMS1516各基因型对90日中国美利奴羔羊体尺的影响较大,其中基因型EF的胸深最大(22.44cm),基因型CD胸围最大(62.280m),管围最大的为CD(8.07cm):微卫星座位OY3不同基因型初生重差异显著(P<0.1),基因型EF的初生重最大(4.10kg),CD基因型初生重最小(3.42kg)。

At the end of 2 months,6 months,9 months,12 months,respectively,left eyeballs of the mice for each group were enucleated to make tissue sections for light study and immunohistochemistry analysis.

取2 月、6 月、9 月、12 月C57BL/KsJ db/db糖尿病小鼠的眼球,制成石蜡切片,行免疫组织化学染色,进行免疫定位及用计算机图像分析仪对视网膜神经节细胞层的AngⅡ进行灰度和面密度分析。

We compared the difference of gene expression between thymus in one-and ten-month old Balb/c micee using DDRT-PCR. It was found that there would be a significant difference of gene expressions in murine thymuses.

我们采用差异显示反转录PCR技术对1月和10月Balb/c小鼠胸腺mRNA的差异表达情况进行了分析,共获得月差异表达的cDNA片段108条(其中90条源于1月小鼠胸腺中,而有18条源于10月小鼠胸腺中)。

Modeling animals: the mortality of 4 weeks old group was 50%,but 6 weeksdidnt die any more; in the 6 weeks group the percentage of CD4. CD8 andthe rate of CD4/CD8 T cell subgroups, the number of lymphocyte inperipheral blood were obviously diminished compared with blank group, hassignificance in statistics(p<0.01); thymuses and spleens were defl- ated in different degree compared with blank group, has significance instatistics.

结果: 1。模型动物的选择:4周组的死亡率为50%,6周组未见死亡;6周模型鼠一般状况较正常鼠明显变差,且外周血淋巴细胞计数及外周血CD4、CD8百分比、CD4/CD8比值较空白组均明显减少,差异均有统计学意义,P<0.05或P<0.01;6周模型鼠胸腺、脾脏均不同程度缩小,与正常比较有统计学意义,P<0.05或P<0.01。

Long-term aerobic exercise can effectively prevent hepatocyte number from decreasing accompanying with aging. Among the groups of aged, results showed that rats of 1-hour and 2hours group whose recovery function of hepatocyte apoptosis were of most significant. The increasing number of hepatocyte might be one of the cytological mechanisms of how aerobic exercise delays cell aging. Further researches on the phenomenon of the anomalous change of the hepatocyte apoptosis ratio among the youth groups are needed expectably.

肝细胞凋亡存在随增明显下降的趋势,这对维持肝细胞数量或缓解增过程中肝细胞数目丢失具有十分重要的意义;长期有氧运动可以有效的阻止肝细胞凋亡率在增过程中下降的趋势;在老年前期组中,训练1小时和2小时组对肝细胞凋亡能力的恢复作用最明显;有氧运动促进肝细胞凋亡能力的提高可能就是有氧运动延缓细胞增发生和发展的重要细胞学机制之一。

The Canadian prime minister makes a special trip to New York, pay respects toward Soong Mei-ling, and invite her to leave for to add.6Month 14 days, Soong Mei-ling leaves for Canadian capital city, Ottawa to do to visit on the 3rd from New York.16The sunrise seat Canadian national legislature deliver a speech, two councilman in the hospitals dedicate with the war whoop with warmly applause.6Month 24 days, Soong Mei-ling once more visits the White House after Canada returned to arrive New York.6Month 29 days, Soong Mei-ling multiplies by an American government to have a special airplane to start on journey to return to country especially from American the south.

加拿大首相专程至纽约,向宋美致敬,并邀请她赴加。6月14日,宋美自纽约赴加拿大首都渥太华做3日访问。16日出席加拿大国会演说,两院议员献以欢呼声与热烈掌声。6月24日,宋美自加拿大回抵纽约后,再度访问白宫。6月29日,宋美自美国南部乘美国政府特备专机启程返国。

The results were as follows. In the insert mutation genotypes, there was significant difference between body weights of 2 weeks aged BB and AB genotype (P.05), while there was no significant difference between AA and other genotypes. In the mutation genotypes, there was no significant difference in body weight. Insert mutation loci had no significant effect on fossil bone length and shank length of 0-12 weeks aged chickens, only mutation loci had a significant effect on fossil bone length and shank length of 6 weeks aged chickens.

分析多态位点与0-12周鸡生长发育性状的关联性,结果表明:插入突变基因型中,2周鸡体重BB型与AB型差异显著(P.05),但AA型与AB型和BB型差异均不显著,而突变各基因型间没有显著差异;插入突变位点对0-12周鸡的龙骨长、胫骨长均没有显著影响,而突变位点仅在鸡6周时表现出与龙骨长、胫骨长间的差异显著性。

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