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The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology. One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension.

本研究用AEV-NH937株组织毒1:100倍稀释,0.03ml/只脑内接种1日SPF雏鸡,分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只,对照观察组2只,分离血清,制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液,同时采集脑组织,10%福尔马林固定,制备石蜡切片,做H.E。

The inactivation of chironomid larvae in water supply systems was experimented with environment degradable vitamin K3,its main component 2-methyl-1,4-naphthoquinone,and their congener menadione triamintriazine bisulphite.

采用易被环境分解的维生素K3、其基本成分β-甲基萘醌和同系物三氨基三嗪甲萘醌亚硫酸盐,对在给水系统中出现的4摇蚊幼虫进行了杀生实验。

Social adjustment is correlated with chronological age and verbal mental age.

DS儿童的社会适应能力与年、智相关。

The concentrations of 17 - KS of 6 to 7 years old normal boys was measured withbiochemistery method, and the relationship between the concentration of 17 - KS and ado-lescence was studied. The concentration of 17 - KS increased with chronological and skeletal age.

我们用生化法对6~17岁男学生17—KS的含量进行了测定,并对17—KS与男性青春期发育的关系作了初步探讨。17—KS含量随年、骨的增加而增加,平均值为10.96mg/24h。

Jejunum and ileum chyme pH had no change with days of age and weaning except that of duodenum.

除十二指肠外,空肠和回肠食糜pH值基本上不受日和断奶的影响。

Cion and rootstock age of grafting Seedling is important to its survival.

接穗与砧木的苗对微嫁接成活率的影响是至关重要的

For this reason, the data suggest that the CIST offers greater overall utility than the SSG for monitoring early-age strength gain of CTB.

基于这个原因,数据表明,中科院提供了更大整体效用较特殊保障监察早期强度增益城巴。

This research investigated the utility of the SSG, CIST, DCP, and FWD for assessing early-age strength gain in CTB.

这项研究调查了公用事业的特殊保障,中科院,磷酸氢钙, FWD的评估早实力获得工商及科技局。

Normal rat embryos and embryos with ARM from gestational days 12.5 to 20 were sectioned serially and sagittally and stained with HE to observe development of cloaca and anorectum continually and contrastively.

12.5天-20天的正常及泄殖腔发育畸形的胎鼠正中矢状面连续切片,HE染色,连续动态对比观察泄殖腔及肛门直肠的发育过程。

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