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Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现,过量表达HmgD引起果蝇幼虫体内血细胞增生,并出现黑色素瘤,为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制,本研究分别提取三中期、后期和相对于野生型化蛹前时刻的幼虫的RNA,首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE,Spn27A在突变体中的转录发生变化,结果发现,Spn27A在三中期的表达量低于野生型,而PPAE的表达高于野生型,表明过量表达HmgD影响了酚氧化酶级联反应途径,低水平的Spn27A不足以抑制PPAE的活性,活化的PPAE催化了PPO的活性,使其转变成有活性的PO,从而促进黑色素的生成,使果蝇体内产生黑色素瘤。

The dietary intake was recorded for 3 consecutive days during the 4th and 6th month of the infants,and the infants were divided into 4 groups with di fferent feeding patterns,namely exclusive breast feeding,breast feeding as the only milk source with other supple- mentary food,mixed feeding(breast and formula feeding plus other supplementary food),and bottle feeding.

记录4、6月连续3d的食物摄入情况,按4月摄入食物种类分为完全母乳喂养(除母乳、水、鱼肝油外不吃其他含热能食物)、不完全母乳喂养(母乳为唯一乳类,同时摄入非乳类辅食)、混合喂养(母乳、配方乳及其他辅食均有摄入)和人工喂养(以婴儿配方乳为唯一乳品,同时也摄入其他辅食)4种喂养方式。

The periosteum of the femoral Metaphysis in aged rat, the RANK mRNA expressed was less than those in the bone of 12-week-old rat, in the trabecular bones, the expressing of RANK was stronger in the ovariectomized rats than in the sham-groups.

在老年雌性大鼠的股骨干骺端皮质骨外膜中RANK的表达较12周鼠的股骨干骺端中RANK的表达减弱,骨内膜中RANK的表达在不同的鼠中变化不明显。

Results The nephrogenic zone appeared in 14th day's kidney of the fetus development, medulla could be found in the late stage of fetus development kidney and developed after birth.

结果 皮质出现的时间为胚14日,早期髓质结构出现在胚第18日。

The simple pigment epithelial cell layer, the optic ventricle, the primitive neuroblastic layer and the peripheral fibrous layer were observed. The number of both the retinal layer and cellular layer increases in the 35 day old Giant panda.

发现胚后64小时大熊猫视网膜的分化程度很低,色素层已形成,但视泡腔明显;神经层由外面数层长梭形、内面数层圆形细胞核及无核的纤维层构成。35天大熊猫视网膜细胞层次增多。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen<1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

采用浸没式膜生物反应器处理焦化废水的试验结果表明:膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力,其去除氨氮的最高负荷为0.19kg/(m3·d),出水氨氮<1mg/L(去除率为99%);泥长可能使微生物的代谢产物或其他大分子物质积累,从而抑制硝酸盐细菌的活性,导致NO2-积累而有利于短程脱氮的进行,但泥过长也会影响亚硝酸盐细菌的活性,从而影响对氨氮的处理效果。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen<1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

简介: 采用浸没式膜生物反应器处理焦化废水的试验结果表明:膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力,其去除氨氮的最高负荷为0.19kg/(m3·d),出水氨氮<1mg/L(去除率为99%);泥长可能使微生物的代谢产物或其他大分子物质积累,从而抑制硝酸盐细菌的活性,导致NO2-积累而有利于短程脱氮的进行,但泥过长也会影响亚硝酸盐细菌的活性,从而影响对氨氮的处理效果。

Isolation and identification results of volatile compounds showed that aldehydes, alcohols and ketones were the major volatile compounds formed in the processing of XUANWEI ham, and had the highest relative contents in the 6-month-old, which were 11326.18, 947.29 and 554.95 μg nonane 100g-1, respectively. The result was relevant to intense hydrolysis of intramuscular fat in the pre-processed ham (4-month-old).

挥发性化合物的分离鉴定结果表明:醛、醇和酮类是火腿加工过程中形成的主要挥发性化合物,在6月时,它们的相对含量最高,分别为11326.18、947.29和554.95μg壬烷/100 g,此结果与加工前期(4月)肌内脂肪发生强烈水解相关。

Methods: 4~6 months old SD female rats were set to be normal control group and 10~12 months old SD female rats whose oestrus elongation were set as primary senile female rat model.

4~6个月雌性大鼠为正常对照组;10~12个月,阴道细胞学表现动情期延长的雌性大鼠作为初老大鼠模型。

The results showed that Lufenuron possessed high ovicidal activity on H.armigera 1-day eggs at 50 mg/L,eggs mortality rate was 87.30%;LC50 of stomach poisoning of 2nd ~ 5st instar larvae were 0.7434 mg/L,1.9669 mg/L,2.0592 mg/L and 2.6945 mg/L with leaf dipping method,respectively.

结果表明,虱螨脲具有较强的杀卵作用,在50 mg/L浓度下,棉铃虫1日卵死亡率达到87.30%;对棉铃虫2~5幼虫具有较高的胃毒活性,其LC50分别为0.743 4 mg/L、1.966 9 mg/L、2.059 2 mg/L和2.694 5 mg/L。

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客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

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