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In this work, laccase was purified from the white rot fungus Cerrena unicolor LS0547 to evaluate its application in dye decolorization.

本文从白腐菌一色齿毛菌LS0547中纯化出胞外漆酶并用于染料脱色实验。

Compared to the other carbon and nitrogen sources studied, sucrose and malt extract were found to be the suitable carbon/nitrogen sources. The decolorization efficiency of Cerrena unicolor Y5002 to orange G was similar at different orange G concentration below 500mg/L; however, alizarin red inhibited the decolorization reaction at the concentration of 400mg/L or above 400mg/L.

对一色齿毛菌Cerrena unicolor Y5002的液体培养脱色条件进行了研究,其最适碳源和氮源分别为蔗糖和麦芽浸粉;在不同橙黄G浓度下均获得较高的脱色率,因此浓度为500mg/L的橙黄G未对该菌的脱色能力产生抑制作用,而浓度为400mg/L茜素红则对其脱色作用产生明显抑制。

Among these strains, Bjerkandera adusta Y5012, Cerrena unicolor Y5002, Funalia trogii Y4997, Trametes suaveolens D8325 and Trametes versicolor Y4946 showed stronger capacity to decolorize the 6 different dyes. The factors related to decolorization of orange G and alizarin red of strain Cerrena unicolor Y5002 were studied by the method of shaking flask.

在40株菌株中,黑管孔菌Bjerkandera adusta Y5012,一色齿毛菌Cerrena unicolor Y5002,硬毛粗盖孔菌Funalia trogii Y4997,香栓孔菌Trametes suaveolens D8325和云芝栓孔菌Trametes versicolor Y4946对刚果红、橙黄G、茜素红、结晶紫、中性红和亚甲基蓝均显示出较强的脱色能力。

Leaf blade abaxially pilose or sparsely pubescent; teeth along bract margins and sometimes along leaf blade margins aristate and with a claviculate apical gland; hypanthium outside basally pilose to subglabrous; sepals margin sparsely ciliate.

叶片背面具柔毛的或疏生短柔毛;沿着苞片边缘和有时齿沿着叶片边缘具芒并且具具卷须顶端腺体;基部在外面的托杯具柔毛的到近无毛;萼片边缘疏生具缘毛。 21 C。 dolichadenia 长腺樱桃

Calyx with 5 equal or subequal teeth, when subequal the lower 2 teeth as long as but narrower than upper 3, all inconspicuously veined; false spikes composed of 2-flowered verticillasters; leaves papery; petiole and stems variously hairy white lanate onl

花萼具5等长或近等长齿,近等长但是比上面的3,全部不明显脉狭窄的下部2齿倍于;由2花轮状聚伞花序组成的假的穗状花序;叶纸质;叶柄和茎各种地有毛的白色的具绵状毛的onl

Basal leaves 2-6, long petiolate; stipules over 1/2 adnate to petioles, broadly lanceolate, membranous, margin sparsely denticulate and ciliate or entire, apex acuminate; petioles 3-9 cm at anthesis, usually green, sometimes purplish, glabrous, nitid, elongated at fruiting to more than 20 cm; leaf blade glabrous on both surfaces or pubescent along veins, 3-sect, segments shortly stipitate, lateral segments deeply 2-fid, central segment deeply 2- or 3-fid, terminal lobes highly variable in shape and size, ovate-lanceolate, lanceolate, oblong, or linear-lanceolate, margin irregularly dentate or shallowly divided, sometimes triparted to pinnatiparted or 3-lobed, apex obtuse or acute.

基生叶2-6,具长叶柄;托叶超过1/2的贴生至叶柄,宽披针形,膜,边缘疏生细齿和具缘毛或全缘,先端渐尖;叶柄3-9厘米在花期,通常绿色,有时略带紫色,无毛,有光泽的,在果期拉长到超过20厘米;叶片两面无毛沿脉具短柔毛,3全裂,裂片具短柄,侧裂片2深裂,中裂片2或3深裂,顶裂片形状和大小极易变,卵状披针形,披针形,长圆形,或线状披针形,边缘不规则牙齿或浅裂,有时3深裂到羽状深裂或3浅裂,先端钝或锐尖。

In China, Pimpinella can be divided into two groups: those species with hairy, puberulent, or distinctly roughened fruits and obsolete calyx teeth; and those with smooth, glabrous fruits and obsolete or conspicuous calyx teeth.

在中国,Pimpinella 可以被分成两组:有毛,被微柔毛的,或果明显粗糙和萼齿废退的;以及那些具平滑,无毛果和废退的或明显的萼齿的。

Calyx ovoid to campanulate, declined in fruit, glandular outside, glabrous or occasionally villous at throat inside, limb 2-lipped; upper lip 3-toothed, middle tooth circular to obovate, margin winged, decurrent, lateral teeth shorter; lower lip 2 toothed, teeth narrower, apex acuminate to spinescent, sometimes approximate.

卵球形的花萼到钟状,在果期下弯,在外面具腺,或偶有具长柔毛在喉部在里面,二唇形的瓣片;上唇具3齿,中间齿圆到倒卵形,具翅的边缘,,侧齿短;狭窄下唇2齿,齿,对具刺,有时紧靠的先端渐尖。

Leaves subsessile or lower ones with petioles 1-2 mm; cauline blade ovate to lanceolate to lanceolate-elliptic, spatulate near base, 1-2.5 × 0.5-1 cm, subglabrous with faintly strigillose margin and midvein, base cuneate to broadly so, margin denticulate with 3-9 teeth per side, apex acute to obtuse.

叶近无柄或下部的具叶柄1-2毫米;茎生叶卵形的到披针形到披针形椭圆,匙形的近基部, 1-2.5 * 0.5-1 厘米,近无毛微弱具短糙伏毛的具边缘和中脉,基部楔形到宽如此,边缘具小齿具齿每边3-9,先端锐尖到钝。

Petiole 8-20 cm, glabrous; leaf blade 5-7-foliolate; leaflets sessile, abaxially green, oblanceolate, 10-25 × 5-12 cm, abaxially glabrous or with sparse whitish hairs, ferruginous tomentose at base and in axils of lateral veins, base cuneate, margin crenate-serrate with teeth crenulate-serrulate, apex acuminate to subcaudate; lateral veins in 18-25 pairs.

叶柄8-20厘米,无毛;叶片5-7具小叶;小叶无柄,背面绿色,倒披针形, 10-25 * 5-12 厘米,背面无毛或者有稀少带白色毛的,被锈色绒毛在基部和在侧脉腋处,基部楔形,边圆齿状锯齿具细圆齿到细锯齿的牙齿,18-25对的侧脉。

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