齐墩果酸

The method was simple and accurate with good reproducibility, and can be used to determine the content of oleanolic acid in Cortex Aralia Elatae.

该方法简单、准确，重复性好，可作为龙芽楤木药材中齐墩果酸的含量测定方法。

Objective To explore the protective effects of oleanolic acid derived from roots and leaves of Aralia Elata Seem, on alcoholic liver disease in rats and its mechanism.

目的：探讨龙牙葱木皂苷－齐墩果酸对大鼠酒精性肝病的防治作用及其机制。

Data aquired from each method are different, and RP-HPLC is the most accurate method for the determination of oleanolic acid in Aralia taibaiensis.

不同方法所得结果差异很大，RP-HPLC法为太白楤木中齐墩果酸含量测定的最佳方法。

In Chapter 1, eleven glycosides (six oleanolic acid saponins, five hederagenin glycosides) were isolated from Decaisnea fargesii Franch.

第一章从木通科猫儿屎植物中分离，鉴定了十一个三萜糖甙化合物，其中六个为齐墩果酸甙，五个为常春藤甙。

Result: 5 compounds were identified as eriodictyol (1), 5, 7-dihydroxy-3′, 4′-dimethoxy flavanone (2), oleanolic (3), 5, 7-dihydroxychromone (4) and homeriodictyol (5) by spectral evidences, in which homeriodictyol (5) had acceleration differentiation to PC12 cell.

结果：获得了5个主要化学成分，即北美圣草素(1)， 5，7-二羟基-3′，4′-二甲氧基-二氢黄酮(2)，齐墩果酸(3)， 5，7-二羟基色原酮(4)，高北美圣草素(5)。经活性筛选试验发现，化合物高北美圣草素(5)具有促PC12细胞分化作用。

objectiveto determine the content of the tritepenoidic acid in dracocephalum moldavica l .methodswith oleanolic acid as the standard,5% vanillin-acetic acid and perchloric as the developer,colorimetric method was applied.

目的测定香青兰全草中总三萜酸成分含量。方法以齐墩果酸为指标，采用香草醛-冰醋酸及高氯酸显色后用分光光度法测其含量。

ConclusionThe method is convenient, fast and reliable and suitable for the quality control of Elsholtzia lychnitis Levl..

该方法简便、准确、快速，可用于三叶香茶菜中齐墩果酸的质量控制。

ObjectiveTo establish an HPLC method for the determination of Oleanolic acid in Elsholtzia lychnitis Levl..

目的建立高效液相色谱法测定三叶香茶菜中齐墩果酸的含量。

Oleanolic acid, mangiferin and swertisin were important bioactive components in Gentianaceae.

采用高效液相色谱法，对青藏高原上9种龙胆科植物中的3种有效成分齐墩果酸、芒果甙和当药黄素进行了分离和分析。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。