齐墩果酸

The method appeared to be simple, accurate and can be used to control the quality of Comus Officinalis Sieb. Et Zuec.

HPLC法可使熊果酸和齐墩果酸达到基线分离，简便准确，可用于药材质量监控。

A method for the determination of the total amount of ursolic acid and oleanolic acid in Cornus officinalis and its steeping wine was developed.

建立了测定山茱萸及其浸泡酒中熊果酸和齐墩果酸总量的方法。

HPLC method was proved to be sensitive, fast and accurate. It can be used for the determination of ursolic acid and oleanolic acid in different colour Rhododendron simsii Planch flowers.

本方法灵敏、准确，重现性好，可用于不同花色映山红花中熊果酸和齐墩果酸含量的测定。

Objective To extract ursolic acid and oleanolic acid from Rhododendron simsii Planch flowers by supersonic technology and develop a RP-HPLC method to determine the content of ursolic acid and oleanolic acid.

目的 结合超声技术提取不同花色映山红花中熊果酸和齐墩果酸，建立RP-HPLC法测定其含量。

The chemical composition research aspect literature report are extremely few: According to reports, the root of Rosa davurica pall includes the lactone, the tanning substance, the return to original state sugar, the total lucoside, the flavanone, the phenol class, the biological alkalis, the protein, the anthraquinone equi com ponent, moreover ,the total polysaccharide is the even polysaccharide which is composed by the fructose.In 1999, for the first time from puncture the root in the alcohol extraction to sep arate Oleanic acid. And used the double wave length thin layer scanning method to determine the uneven pillar tartaric acid in to puncture in the root ontent.

化学成分研究方面文献报道极少：据报道，山刺玫根中含有内酯、鞣质、还原糖、总苷、黄酮、酚类、生物碱类、蛋白质、蒽醌等成分，而且山刺玫根中总多糖是由果糖组成的均多糖。1999年，首次从刺玫根的乙醇提取物中分离出齐墩果酸，并采用双波长薄层扫描法测定了齐墩果酸在刺玫根中的含量。

Objective To establish a method for determining the content of oleanolic acid and ursolic acid in Prunella vulgaris.

目的 建立夏枯草中齐墩果酸和熊果酸的含量测定方法。

This method is reliable,rapid and accurate, which could be used for the quantitative analysis of the contents of oleanolic acid and ursolic acid in Prunella vulgaris.

该法简便、准确、快速、重现性好，可用于夏枯草药材中齐墩果酸和熊果酸的含量测定。

On the basis of spectral analysis, these compounds were identified as Kaempferol , Kaempferol-3-O-β-D-glucopyranoside , Kaempferol-3-O-β-D-galactopyranoside, Quercetin , Quercetin-3-O-β-D-arabinopyranoside, Quercetin-3-O-β-D-galactopyranoside, gallic acid, methyl gallate-4-O-β-D- galactopyranoside, caffeicacid, methyl caffeate, 4-cafeoyl-(3"a, 4"a, 5"β)- trihydroxyquinic acid, 3"-cafeoyl-(3"a, 4"P, 5"P )-trihydroxyquinic acid methyl ester, 5"-cafeoyl-galactonic acid , pumiloside, loganic acid , 5R,6S,7E-megastima-3-on-7-en-9-ol 9-O-β-D-glucopyranoside. 2a, 3β-dihydroxy-oleanoic acid , 2a, 3β, 23-trihydroxy-oleanoic acid, 3a, 6a-dihydroxy-oleanoic acid, 3β-hydroxy-ursoic acid, 2a, 3a, 23-trihydroxy-ursoic acid , 2a, 3β, 23-trihydroxy-ursoic acid, 2β, 3β, 19a -trihydroxy-ursoic acid, 2a , 3β, 19a -trihydroxy-ursoic acid-28-O-β-D-glucopyranoside , 2a. 3a, 19a-trihydroxy-ursoic acid-28-O-β-D-glucopyranoside, 2a, 3a, 19a, 23-tetrahydroxy-ursoic acid, 1a, 3a, 19a, 23-tetrahydroxy-ursoic acid-28-O-β-D-glucopyranoside , 3a, 19a, 23-trihydroxy-ursoic acid.

从珙桐中分离鉴定得到了28种化合物，它们分别是：山奈酚，3-O-β-D-吡喃葡萄糖基-山奈酚甙，3-O-β-D-吡喃半乳糖基-山奈酚甙，槲皮素，3-O-β-D-吡喃阿拉伯糖基-槲皮素甙，3-O-β-D-吡喃半乳糖基-槲皮素甙，没食子酸，4-O-β-D-吡喃半乳糖基-没食子酸甲酯，咖啡酸，咖啡酸甲酯，4-咖啡酰基-(3&α，4&α，5&β)-三羟基鸡纳酸，4-咖啡酰基-(3&α，4&β，5&β)-三羟基鸡纳酸甲酯，5'-咖啡酰基-半乳糖酸，pumiloside，loganic acid，5R，6S，7E-megastima-3-on-7-en-9-ol9-O-β-D-glucopyranoside，2α，3β-二羟基齐墩果酸，2α，3β，23-三羟基齐墩果酸，3α，6α-二羟基齐墩果酸，3β-羟基熊果酸，2α，3α，23-三羟基熊果酸，2α，3β，23-三羟基熊果酸，2β，3β，19α-三羟基熊果酸，2β，3β，19α-三羟基熊果酸-28-O-β-D-吡喃葡萄糖甙，2α，3α，19α-三羟基熊果酸-28-O-β-D-吡喃葡萄糖基甙，2α，3α，19α，23-四羟基熊果酸，2α，3α，19α，23-四羟基熊果酸-28-O-β-D-吡喃葡萄糖基甙，3α，19α，23-三羟基熊果酸。

Results indicate that the optimum extraction conditions are as follows: ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8︰1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 mL/min; the desorption solvent is 90% ethanol, and the flow rate of elutant is 1.0 mL/min.

结果表明，超声波辅助提取最佳工艺条件为：超声功率600 W、提取温度53℃、液料比8：1、超声波提取70 min，回流提取120 min，齐墩果酸得率为3.000 mg/g；XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果，最佳纯化工艺条件为上柱液浓度0.794 mg/mL，吸附流速0.5 mL/min；以90%浓度乙醇洗脱，洗脱流速1.0 mL/min，纯化后得提取物中齐墩果酸纯度可达26.55%。

ATA was found to exhibit more distinctive cytotoxicity toward human cervical squamous carcinoma HeLa cells than oleanolic acid, which suggested that the position of the carboxyl group significantly affects the cytotoxicity of oleanane-type pentacyclic triterpenes with a carboxyl group.

周晓华被发现展品更为鲜明的细胞毒性，对人体颈部鳞癌Hela细胞比齐墩果酸，这表明的立场与羧基显着影响细胞毒性的齐墩果烷型pentacyclic三萜类化合物与羧基。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。