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MAIN OUTCOME MEASURES: The contents of monoamine neurotranmitters of norepinephrine, adrenalin, dopamine and 5-serotonin (5-HA) and its metabolites of DHPR, 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid in the striatum and marginal area of brain in rats were observed.

主要观察指标:大鼠纹状体和脑边缘区单胺类递质去甲肾上腺素、肾上腺素、多巴胺和5-羟色胺及其代谢产物3,4-二羟苯乙二醇、5-羟吲哚乙酸、高香草酸的含量。

This suggested that 5-HT contents were high correlated with the presence of symptoms and inflammation in human pulpitis.

经色谱分析提示,人和大鼠牙髓中5-HT的来源和存在形式有所不同,其代谢产物均为5-羟吲哚乙酸。

Methods The tetramine samples in 0.5 μg/mL concentration were stored in the environment temperature at 45 ℃, 25 ℃ and 4 ℃. The tetramine concentration were measured by GC-MS in 1, 3, 12, 18 and 39 days, respectively. And then the results of measurement were analyzed by SPSS software.

配制毒鼠强质量浓度为0.5 μg/mL的血液样品,分别置于45 ℃、25 ℃和4 ℃环境中保存,在配制当天及存放3、12、18和39 d用气相色谱-质谱联用法测定其质量浓度,运用SPSS统计软件对结果进行统计分析。

Five rats from both groups were killed at days 7,14 and 28,respectively.And we used deproteinization analysis to detect the plasma H2S concentration and hematoxylin and eosin stain and Mallory trichrome stain for the lung tissue.

观察指标:于试验的第7、14、28天时每组分别处死5只大鼠,采用去蛋白分析方法测量血浆中H2S含量,取肺组织行HE染色和Mallory三色染色。

for the determination of the asiaticoside metabolite asiatic acid and ursolic acid was developed.

建立大鼠血浆积雪甙代谢产物积雪酸的气相色谱质谱检测方法。

Most 5-HT cells appeared in the base part of gland in the rat, while in the human they were mainly located in the intercellular substance and a few scatted in the glandular epithelium.

方法采用免疫细胞化学方法,检测人和大鼠胃窦部粘膜内生长抑素细胞、胃泌素细胞、5-羟色胺细胞(5-HT细胞)、嗜铬粒素A细胞的分布。

Successfully operated rats were parted into 2 groups: model group and sappan group. Transmigrated constituents of sappan in transplanted hearts were observed by HPLC-FPC.

通过反相HPLC色谱分析方法,检测苏木乙酸乙酯提取物在心脏移植大鼠供体心脏中的分布代谢情况,并观察移植心肌组织病理形态学改变。

Results: 1 The analysis revealed that GAP-43 immunopositive neurons in rats were localized in all the layers of lateral geniculate nucleus and the layers II -VI of visual cortex area 17,and GAP-43 is stained by a ring and dot figure and brown-yellow colour in cytomembrane ; 2, In the critical period, gene expression concentration and number of GAP-43 immunopositive neurons, in both the lateral geniculate nucleus and the visual cortex area 17, received from the deprived eyes, are reduced.

结果:1、GAP-43在正常大鼠视觉系统表达主要见于外侧膝状体全层和视皮质17区Ⅱ—Ⅵ层神经元胞膜中,呈环状或点状棕黄色免疫阳性反应;2、在敏感期内剥夺眼对侧外侧膝状体和视皮质17区GAP-43表达的免疫阳性神经元染色变淡(P<0.05),且免疫阳性神经元数目减少(P<0.05)。

Methods Immunohistochemical method was used and the depletion area of CTnT in myocardial ischaemia were measured by the computer image analysis.

应用免疫组化LSAB法显色观察。结果缺血15分钟,大鼠心肌肌钙蛋白T即有明显脱失,随缺血时间的延长,脱失面积呈扩大趋势。

Paclitaxel concentration in plasma, pelvic lymph nodes, liver, heart were determined by high-performance liquid chromatography.

以高压液相色谱方法检测大鼠血浆及肝、心、肿瘤组织及盆腔淋巴结中的药物浓度。

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