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The genistein and glucuronidated genistein in plasma and in different tissues were detected by a high performance liquid chromatography ashy.

用反相高效液相色谱法测定不同时间大鼠血浆中原型药物、β-葡萄糖醛酸结合型药物及组织器官中药物的浓度。

(1) to deliver the Levonorgestrel (0.ml/kg,5mg/m) and Mifepristone gelatum (0.1ml/kg,5mg/ml) per cutem to the rat respectively and measure the serum concentration of levonorgestrel、mifepristone、estradiol and progesterone at different time by the means of HPLC, radioimmunoassay,chemical separation and atomic absorption spectrophotome.

大鼠皮肤涂抹米非司酮凝胶(0.1ml/kg,5mg/ml)和左炔诺孕酮凝胶(0.1ml/kg,1mg/ml)后的不同时间,利用高效液相色谱法、放射免疫分析法、化学分离与原子吸收分光光度法,测量血清中米非司酮浓度、左炔诺孕酮凝胶以及血清雌二醇、孕酮浓度。

The HPLC finger print of TGDGBX was set up by HPLC-DAD: the chromatographic column,Diamonsil C18(250 mm×4.6 mm, 5μm, 30℃)and bidimensional gradient elution using methyl cyanide-water (flowing speed 1.0 mL·min-1,λ=203 nm). 180 Wistar rats were randomly divided into normal control group, model group, cortisone group, and TGDGBX (16,32 and 64 mg·kg-1) groups respectively.

采用HPLC-DAD,Diamonsil C18色谱柱(250 mm×4.6 mm, 5μm),乙腈―水二元梯度洗脱,流速1.0 mL·min-1,(λ=203 nm),柱温为30℃,建立了当归补血总苷的HPLC指纹图谱。180只Wistar大鼠随机分为正常对照组、模型对照组、强的松组和当归补血总苷大、中、小剂量组,每组30只。

ObjectiveTo observe the effect of borneol on synthesis and transmission of 5-HT in rat different brain regions.

目的观察冰片对大鼠不同脑区5-羟色胺(5-HT)合成及转运的影响。

Results Administration of L-NAME could attenuate the activity of NOS and inhibit spermatogenic cells apoptosis in rats experimental cryptorchidism, and it could significantly inhibit weight loss of the cryptorchid.

手术建立大鼠单侧隐睾模型,实验分为隐睾组、隐睾+L-NAME组[术后腹腔注射溶于生理盐水的L-NAME 50 mg/(kg\5d)]、隐睾+生理盐水组、假手术组,7 d后采用化学比色法检测睾丸组织NOS活性,流式细胞术和原位缺口末端标记法检测生精细胞凋亡,RT-PCR和免疫组织化学法检测Bax基因表达变化。

Results ITF and ITF mRNA were expressed in whole small intestine and most of them were localized in goblet cell of intestinal villus.

在此基础上采用原位杂交、免疫组化和高效液相色谱等手段观察了烧伤后两组大鼠ITF 及ITF mRNA的变化。

The Morris Water Maze experiment was used to measure the learning and memory abilities. The content of ACH was determined by alkaline hydroxylamine.

水迷宫实验观察大鼠学习记忆能力的改变;碱性羟胺比色法测定海马乙酰胆碱含量。

For example, in adult control offspring, densities of 5-HT transporters were significantly greater in the cingulate cortex (+33%), basolateral amygdala (+58%), and CA1 area of the hippocampus (+78%); but significantly lower in the temporal cortex (-65%) and median raphe (-25%).

比如,在对照的成年大鼠,5-羟色胺转运体密度在下列区域显著地高:扣带回皮质(+33%),底侧杏仁核(+58%),海马CA1区域(+78%),但在颞叶(-65%)和内侧缝核(-25%)显著地低。

Objective To evaluate the effects of selective serotonin reuptake inhibitors on sleep apneas in Sprague-Dawley rats.

目的 研究选择性5-羟色胺再摄取抑制剂帕罗西汀对SD大鼠睡眠呼吸暂停的影响。

Objective To develop a rapid approach of refractive index detection with high performance liquid chromatography for the determination of rhamnose, fructose, glucose, surose and maltose in Lycium barbarum L.

目的 建立同时测定枸杞中鼠李糖、果糖、葡萄糖三种单糖和蔗糖、麦芽糖两种低聚糖含量的高效液相色谱-示差折光检测方法。

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