鼠色的

Methods: Adopting pig bile culture medium, the inhibiting or killing effects on pig bladder worm in vitro were observed;by using the methods of xylene-induced ear inflammation in mice and granuloma swelling induced by cotton ball in rats, the effects of anti-inflammation were observed;by using the methods of body twisting and hot-plate in mice, the effects of analgesia were observed. Through the mice auricle microcycle, the rats thrombus law in vitro and bleeding time, clotting time determination to the mice, the function of invigorating the blood were observed;adopting the penicillin abdominal cavity injection method, the mice epilepsy model were prepared, and epilepsy mice brain organization MDA content and SOD, ATPase vigor be measured by using chemical colorimetry.

利用猪胆汁培养基观察该方在体外对猪囊尾蚴的抑制或杀灭作用；采用二甲苯所致小鼠耳肿胀法、大鼠棉球肉芽肿法以观察该方的抗炎作用；采用热板法和化学刺激扭体法观察该方的镇痛效果；通过小鼠耳廓微循环法、大鼠体外血栓法和对小鼠出、凝血时的测定观察该方的活血作用；采用青霉素腹腔注射法制备小鼠癫痫模型，并利用化学比色法检测癫痫小鼠脑组织中MDA的含量及SOD、ATPase的活力。

The neurological functional recovery of stroke rats is assessed by Longa 5 grades. Sucrose solution consumption test and open-field test are used to estimate the change of interest, the level of spontaneous motor activities and investigative behavior of PSD rat model. Fluorophotometry is applied to detect the monoamine neurotransmitters(NE 5-HT and DA) of the double frontal-parietal cortexes and brain stem.

运用Longa 5分法评定卒中大鼠神经功能；运用糖水消耗实验、敞箱实验评定PSD大鼠的抑郁行为学改变；采用荧光分光度法测定双侧额顶皮层及脑干的单胺递质：去甲肾上腺素、5-羟色胺(5-HT)、多巴胺的含量。

Anthocyanidin extract in methanol showed 3 absorption peaks, at 270, 340 and 520 nm, in the UV-vis spectrum, with bathochromic and hypsochromic shift with methanol+AlCl3 and methanol+AlCl3+HCl, respectively; the ratio of the absorbance at 440 nm to that at 520 nm was 0.29. No fluorescence occurred under UV irradiance, and 5 absorption peaks occurred at 270 nm after HPLC. Five molecular ion peaks occurred on MS: 595, 381, 611, 587 and 571, respectively, and monosaccharides in the extract were identified as rhamuose and glucose on HPLC.

一品红花色素的甲醇溶液分别在270、340 和520 nm 处有 3 个吸收峰；在 440 nm 吸光度与可见光最大吸收波长 520 nm 吸光度的比值为0.29；花色素的甲醇溶液中加入AlCl3后发生红移，再加入HCl后发生蓝移；色素溶液在紫外光下无荧光；色素样品经液相色谱分离后在 270 nm检测有 5 个比较明显的吸收峰；质谱中得到 595、611、381、571 和 589 等对应的分子离子峰；花色素酸解液高效液相色谱图谱和鼠李糖、葡萄糖的出峰时间一致。

Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.

本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的，对其化学成分进行研究，采用70％甲醇冷浸的方法对刺五加花蕾中的成分进行提取，运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离，得到5种单体化合物，分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E；利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定；考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况，并作一比较，为今后建立刺五加质量评价体系提供了科学依据，奠定了坚实的基础。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Scandent shrubs or lianas; leaf blade ferruginous villous abaxially, glabrous adaxially, except midrib and lateral veins ferruginous strigose; flower bisexual; petals subspatulate, 2 × length of sepals, apex conspicuous.

攀缘灌木或者藤本植物；背面的叶片具铁锈色长柔毛，正面无毛，除铁锈色具糙伏毛中脉和侧脉之外；花两性；近匙形的花瓣，2 *萼片的长度，先端明显。 1 D。 longipetalum 海南毒鼠子

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种，接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况；并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

OBJECTIVEO To study the changing of bulbus medullae SP and 5-HT when rats were disposed by different medicine that can make people vomit and provide experimental basis for detecting the pathological mechanism of reverse ascending of Stomach—Qi.

目的：通过对大鼠给与不同致呕吐物质的刺激，研究大鼠在接受不同呕吐物质刺激后其延髓组织P物质及5-羟色胺(5-HT)的变化，为中医学&呕吐&证发生的病理病机&胃气上逆&的理论，提供分子生物学依据。

In the present study, the effects of GS on learning, memory, activity and serotonin of CNS of rats were explored under different sleep deprivations.

本课题主要研究连续口服人参皂贰对睡眠剥夺造成的大鼠学习、记忆和情绪兴奋性损害的保护作用，并探讨不同睡眠剥夺时间长度下，人参皂成对大鼠中枢五羟色胺的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。