鼠色的

The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%，主要为是I型、III型胶原蛋白，本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白，将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解，液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽，利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

We investigated constituent of triterpenoid saponins ofAlbizzia, two new compounds together with two known compounds were isolated from Albizzia julibrissin Durazz. by using column chromatography (macroreticular resin, silica gel, Sephadex gel, reverse phase silica gel),preparative HPLC methods et al.On the basis of spectroscopic analysis, including IR,ESI-MS,~1H-NMR,~(13)C-NMR,HMBC,HMQC,~1H-~1HCOSY and chemical methods, the structure of two new compounds were identified as 3 - O -[β-D-xylopyranosyl(1→2)-β-D-fucopyranosyl (1→6)-β- D -2- deoxy - 2 - acetoamidoglucopyranosyl] -21-O-[(6S)-2- trans- 2,6-dimethyl - 6 - O-β- D - quinovopyranosyl -2,7- octadienoyl] - acacic acid- 28 - O-β-D-glucopyranosyl(1→3)[α-L-arabinofuranosyl(1→4)]-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside acacic acid 3- O -β- D- glucopyranosy(1→3)-β- D- fucopyranosl(1→6) [β-D- xylopyranosyl (1→2)]-β-D-glucopyranoside ;two known compounds were acacic acid lactone 3- O -β-D- xylopyranosyl-(1→2)-β-D-fucopyranosl (1→6)- 2-deoxy -2 -acetoamido -β-D- glucopyranoside ; acacic acid lactone 3- O-β-D-xylopyranosyl(1→2)-α-L- arabinopyranosl (1→6)- 2- deoxy - 2- acetoamido -β-D-glucopyranoside . The study lays chemical foundation and chemical reference substance for enhancing quality standard of Albizzia julibrissin Durazz.

本研究论文在综述国内外对合欢属Albizzia三萜皂苷化学成分和药理作用研究进展的基础上，利用传统植化分离手段和现代分离技术，包括大孔树脂、硅胶、葡聚糖凝胶、反相硅胶等柱色谱，制备高效液相色谱法等技术从中药合欢皮中分离得到了4个化合物，其中，2个新化合物和2个己知化合物，并进一步通过现代分析技术IR，ESI-MS，~1H-NMR，~(13C-NMR，HMBC，HMQC，~1H-~1HCOSY等和化学方法鉴定了2个新化合物的结构分别是：3-O-[β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖基]-21-O-[(6S)-2-反式-2，6-二甲基-6-O-β-D-吡喃鸡纳糖基-2，7-辛二烯酸基]-金合欢酸-28-O-α-L-呋喃阿拉伯糖基(1→4)[β-D-吡喃葡萄糖基(1→3)]-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷，金合欢酸3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃夫糖基(1→6)[β-D-吡喃木糖基(1→2)]-β-D-吡喃葡萄糖苷；2个已知化合物结构分别是：金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷，金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-α-L-吡喃阿拉伯糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷。

Methods Animal inflammatory models were established by injection of dimethylbenzene for mice ear swelling, carrageenin for rat footpad, cotton pellet wool for rat granuloma respectively. The content of prostaglandin E2(PGE2) and leukotriene B4(LTB4) from peritoneal macrophages were measured by RIA and RP-HPLC respectively.

采用二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足肿胀、棉球诱导的大鼠肉芽肿模型观察TFC的抗炎作用；分别采用放射免疫法和反相高效液相色谱法检测TFC对大鼠腹腔巨噬细胞产生前列腺素E2(PGE2)和白三烯B4(LTB4)的影响。

Methods:Thirtytwo rats were randomly divided into four groups(normal control group,celiac seawaterimmersing trauma stress 1,2,3h groups).The amount of hexosamine and phospholipids in gastric mucosa of rats were detected by both chemochromometric and modified chemical methods.

32只SD大鼠随机分成正常对照组和腹腔浸泡伤1h、2h、3h 4组，分别取相应大鼠的胃黏膜，采用化学比色法和改良抗坏血酸法测定胃黏膜中氨基己糖和磷脂含量。

The subacutely aging rats were made by injecting Dgal into the rats' abdominal cavity continuosly. The learning and memory ability of rats in each group were detected with Morris water maze. The monoamine transmitter contents in hypothalamus and hippocampus were detected with HPLCECD.

D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型，采用Morris水迷宫检测各组大鼠的空间学习记忆能力及采用高效液相色谱电化学法检测各组大鼠下丘脑、海马NE，DA，5HT的含量。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

And 5-hydroxytryptamine (5-HT) in rat nucleus accumbens and ventral tegmental area.

高效液相色谱-电化学法测定大鼠伏隔核及腹侧被盖区内去甲肾上腺素、多巴胺和5羟色胺(5HT)的含量。

Then all the rats were sacrificed after body weighing. The liver and splenic indices were calculated. HE and Masson's trichrome staining were used to evaluate the degree of liver inflammation and fibrosis. The ultrastructure of hepatocytes was observed by transmission electron microscope.

实验结束后称量大鼠体重，处死大鼠，计算肝、脾指数；HE和Masson三色染色评价肝组织炎症和纤维化程度；透射电镜观察肝细胞超微结构的变化。

objectiveto study the effects of glycyrrhizin on intestinal absorption of paeoniflorin by the everted rat gut sac model.methodsused a simple rp-hplc method for the determination of paeoniflorin in the krebs fluid.

目的研究甘草酸对芍药苷大鼠肠吸收的影响。方法采用大鼠肠外翻模型研究芍药苷的肠吸收，并用反相高效液相色谱法测定大鼠肠黏膜内外两侧的芍药苷浓度。

The capability limens of the sacculus that caused abdomen-uplifting and back-arching and the times of contract of abdomen muscle in rats under different dilatations were observed. The levels of 5-hydroxytryptamine (5-HT), substance P, and calcitonin gene related peptide were evaluated.

观察各组大鼠肠道内扩张引起腹部抬起和背部拱起的容量阈值和大鼠肠道内不同容量下扩张期间腹壁收缩次数，检测5-羟色胺(5-HT)、P物质、降钙素基因相关肽水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。