鼠毒

Because snails were eliminated when we poisoned fish in brooks and mice in houses or fields(mice are the final or transfer hosts of Paragonimus ) and mammals such as cats and dogs died by eating poison or poisoned fish or mice, the infection rate of P.

卫生并殖吸虫疫区感染率下降主要与溪河毒鱼时殃及螺蟹，房屋与田野毒鼠（并殖吸虫终末宿主或转续宿主）及致猫犬科动物误食毒鼠药饵或中毒的鱼、鼠致死所致。

Results In the brornadiolone group, the coefficients of the bait intake were 1.05 and 2.89 for the mice and rats respectively, and were 0.81 and 0.32 for cournatetralyl.

结果 大白鼠、小白鼠对0.005％溴敌隆的再遇摄食系数分别为1，05和2.89，毒杀率均为100％；大白鼠、小白鼠对0.0375％杀鼠迷的再遇摄食系数分别为0.81和0.32，毒杀率分别为100％和91.67％。

Objective To study the relationship between formation of toxemia and oxidative damage of central nervous system of mice with trichinosis.

目的 探讨旋毛虫病鼠毒血症的形成以及与中枢神经系统氧化损伤的关系。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

Bone marrow transplantation + habu snake venom group: recipients accepted habu snake venom through tail veins five weeks after transplantation and were provoked mesangio proliferative glomerulonephritis, then observed the green fluorescence in recipients kidneys on the 7, 14, 28, 56 day after habu snake venom injection.

骨髓移植+竹叶青蛇毒组：受体鼠在接受骨髓移植后的五周通过尾静脉注射竹叶青蛇毒诱发系膜增生型肾小球肾炎，竹叶青蛇毒注射后的第7、14、28、56天观察受体鼠肾脏中的绿色荧光。

Whole-cellpatch-clamp technology demonstrated that VDPG (1g/L) had notsignificant effects on the delayed-rectified K~+ current, TTX-sensitive Na~+ current and high-voltage-activated Ca~(2+) current of rat dorsalroot ganglion cells. The fast transiet K~+ current of cottonbollworm dorsal DUM cells, the fast transiet K~+ current, Na~+ current andhigh-voltage-activated Ca~(2+) current of Periplaneta Americana dorsalunpaired median cells were also not significantly affected byVDPG at the same concentration. However, VDPG had significant effecton the fast transiet K~+ current of Pieris rapae. The VES had not significanteffects on the high-voltage-activated and low-voltage-activated Ca~(2+)current of rat DRG cells.

膜片钳电生理实验显示1g／L毒囊粗毒对蜚蠊DUM神经元的快瞬时钾电流、钠电流、高电压激活的钙电流，对棉铃虫快瞬时钾电流和大鼠DRG细胞延迟整流钾电流、TTX-S型钠电流、高电压激活的钙电流均无明显作用，却对菜青虫快瞬时钾电流有明显作用；电刺激粗毒对大鼠DRG细胞低电压和高电压激活的钙通道无明显作用。

PTX at tenuates the ALI induced by LPS or sepsis in rat. The therapeutic effects of PTX include:improve oxygenation, at tenuate pulmonary edema formation, enhance animal survival in sepsis-induced ALI. The mechanisms may related with the effects of PTX inhibiting the chemotaxis, migration, activation of inflammatory leukocytes, diminishing the production of active oxygen, and inhibiting inflammatory cytokine release partly.

4PTX对大鼠内毒素性ALI及脓毒症性ALI均有一定的防治作用，主要表现在改善动脉血氧合，减轻肺水肿形成，提高脓毒症性ALI大鼠的存活率等方面，其机理可能与抑制白细胞趋化与激活，减少肺内白细胞渗出及活性氧产生，一定程度上抑制炎性细胞因子生成等有关。

Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly的基因工程毒之间并无显著差异。

Methods Mousetraps were distributed for the investigation; direct immune fluorescence was applied to detect the virus carriage in rats, 3451 mousetraps were distributed and 886 rats were caught, the rats density was 25.67%; 50 caught rats carried EHFV virus (5.64%) It has been proved that there exists a natural cycle of EHFV virus in Kaifeng County; effective measures should be taken to control the epidemic situation of EHFV.

用布夹法开展鼠密度调查，用直接免疫荧光技术进行鼠肺带毒检查。结果共有效布放鼠夹3451夹次，捕获鼠886只，鼠密度为25.67%。带毒50只，鼠类带毒率为5.64%。捕获鼠种有大家鼠、小家鼠、黑线姬鼠、大仓鼠和小仓鼠。结论证实河南省开封县存在肾综合征出血热病毒的自然循环，应采取有效措施控制流行性出血热疫情。

The therapeutic efficacy of hemoperfusion and dimerca prol in rescuing patients with acute tetramine poisoning is better than dimercaprol and routine therapy only.

结论HP联合二巯基丙醇救治急性毒鼠强中毒临床疗效优于单纯二巯基丙醇和常规救治措施，可以明显降低患者B—EP、ET、NO与TNF水平，可作为治疗急性鼠毒强中毒的一种较为理想方案。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。