黏酸的

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Lactobacillus acidophilus had antagonism effect on cell adherence of helical and coccoid forms of Hp.

嗜酸乳杆菌对不同形态的Hp均具有黏附拮抗作用。

The dilational viscoelastic properties of partly hydrolyzed polyacrylamide and alkyl benzene sulfonate in the absence or presence of electrolyte were investigated at the decane water interface by means of longitudinal method and the interfacial tension relaxation method.

采用小幅低频振荡和界面张力弛豫技术，研究了部分水解聚丙烯酰胺(Mo-4000)和阴离子表面活性剂2-丙基-4，5-二庚基苯磺酸钠(377)体系在癸烷/水界面上的扩张黏弹性质，并考察了电解质对体系界面流变性质的影响。

RESULTS: Islets isolated and digested were stained red by dithizone and no stain was observed in the islet exocrine gland. The purity of islets was ≥ 85%. The results of MTT and functional detection showed that the survival rate of islets in the scaffold group was significantly higher than control group (P 0.05). The observation under scanning electron microscopy demonstrated that islets tightly adhered to the PGA scaffolds and grew in the three-dimensional status.

结果：分离和消化后的胰岛被双硫腙染成红色，胰岛外分泌腺不着色，胰岛纯度≥85%；MTT及胰岛功能检测结果显示，支架组的胰岛成活率及胰岛功能明显增高，与对照组相比差异有显著性意义(P 0.05)；扫描电镜观察，胰岛紧密黏附在聚乙醇酸支架上，支架上胰岛成三维立体生长。

Results Ketoprofen calcium pectinate gel beads and calcium alginate gel beads showed excellent drug release pattern and mucoadhesiveness. Ketoprofen released from calcium pectinate beads mainly through diffusion behaviors, whereas the release of ketoprofen from calcium alginate gel beads was ruled by corrosion of carrier material.

结果 酮洛芬果胶钙凝胶小球和酮洛芬海藻酸钙凝胶小球均具有良好的生物黏附性能，果胶钙凝胶小球主要通过溶胀作用缓慢释药，而海藻酸钙凝胶小球的释药与凝胶小球慢慢吸水后骨架溶蚀有关。

Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope.

将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上；MTT法检测支架对人牙周膜细胞黏附、生长的影响；并用倒置相差显微镜和扫描电镜观察形态变化。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

Spinal cord injury models were made in 60 wistar rats and the dynamic expression of bromodeoxyuridine and polysialylated ependymal cells adhesion molecule were determined by immuno-histochemisty.

方法］Wistar大鼠60只，制作大鼠脊髓全横断损伤模型，根据伤后不同时间点分组，应用免疫组织化学方法动态检测脊髓内5-溴脱氧尿嘧啶、多唾液酸神经细胞黏附分子的表达。

AIM: To observe the immune property of cervical carcinoma and antitumour effect of heat-killed lactobacilli adhered to HeLa cells.

目的：比较热灭活嗜酸乳杆菌黏附于宫颈癌HeLa细胞后对肿瘤细胞免疫学性状的影响。

A novel microemulsion was prepared to increase the solubility and enhace the in vitro transdermal delivery of OXA. For the microemulsions, types of oil phase, surfactants, cosurfactant were chosen and the double-distilled water was purified as water phase.

奥沙普秦玻璃酸钠凝胶的流变学实验结果显示，药物玻璃酸钠凝胶具有假塑性流体特性，在低剪切频率时具有较高的黏度，而在高剪切频率时具有较低的黏度，有利于制剂在皮肤表面的涂布和黏附。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐