黏菌

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Mltrastmcture of the interaction showed Cryptococcus neoformans could survive and bud in the cell.

结论活的隐球菌黏附与侵袭肺泡上皮细胞是隐球菌感染肺部的重要条件，不同变种对肺部的易感性可能不存在差异。

Researchers have found that pilus is related to the adhesion and colonization of V.cholerae..Mannose-Sensitive Hemagglutinin belongs to the members of type IV pilus,which can determine its activity of hemagglutination..

国内外不少学者发现，菌毛与霍乱弧菌的黏附定居有关，其中甘露糖敏感血凝素属于Ⅳ型菌毛，决定Ⅳ型菌毛的血凝活性。

These data indicate that hCD46 is a kind of crucial pilus receptor that mediates the host specific adhesion by N. gonorrhoeae, and the hCD46 minigene, pCD46, can be used to develop transgenic mice model for the study of gonococcal diseases.

提示hCD46是菌毛介导的淋病奈瑟菌特异性黏附于人黏膜细胞的一种重要受体，hCD46小基因可用于淋病奈瑟菌感染转基因小鼠模型的制备。

Results There existed the correlation between atlE gene expression and the quantity of extracellular DNA re leasing into the medium, and DNase Ⅰ affected biofilm formation and reduced initial adherent capacity of S.

结果 表皮葡萄球菌atlE基因的表达与胞外DNA的释放量有相关性；DNA酶能影响未成熟生物膜且能降低起始黏附能力；ΔatlE菌株胞外DNA的释放减少，起始黏附能力明显降低。

To understand the role of the mucosa-associated microbiota in pathogenicity of ulcerative colitis, paired biopsies were obtained during colonoscopy from the ulcerated and nonulcerated gut mucosa of 24 patients with UC. The dominant bacteria and four important groups lactic acid bacteria, Clostridium leptum subgroup, Bacteroides spp.

通过PCR-DGGE技术比较24例溃疡性结肠炎患者肠道内溃疡粘膜以及与溃疡粘膜相距5cm的非溃疡粘膜所黏附的优势菌以及拟杆菌、柔嫩梭菌、乳酸菌和双歧杆菌的组成情况，结果表明同一肠段两个部位的优势菌组成很相似，而柔嫩梭菌及乳酸菌的组成存在很大的差异。

To investigate the relationship of subunit of F18 fimbrial adhesin with the adhesion effect,adhesive inhibition tests were performed using antibodies specific for the subunits FedA,FedE and FedF.

利用抗大肠埃希氏菌F18菌毛主要结构亚单位FedA特异抗体和次要结构亚单位FedE、FedF特异抗体，研究了F18菌毛与小肠上皮细胞的黏附特性。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Methods The AGS cellseeded coverslips were prepared, then the two different status of Lactobacillus acidophilus and two different forms of Hp were added in different order. 2 hours later, the bacteria were Garm's stained, and the adherence of Hp to AGS cell were observed by microscope and the adherence index was calculated.

制备人胃上皮腺癌细胞系细胞爬片，将两种状态嗜酸乳杆菌和不同形态Hp菌分组按不同顺序加入，作用2 h，革兰染色后光镜观察分析Hp黏附指数产生的变化，荧光抗体染色法定性评价黏附于AGS细胞的Hp密度的改变。

The protection of the mAb was analyzed via the inhibition of the mAb to the germ tube formation and the adhesion of C.albicans to the epithelial and endothelial cells.

在白念珠菌芽管形成条件下，加入mAb，观察其对白念珠菌芽管诱导的抑制及白念珠菌对上皮细胞、内皮细胞黏附的抑制。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。