黏菌

Agathidium bushi, a beetle that feeds on slime-mold, was named last year after US President George W. Bush.DPA

去年美国总统布希的名字才被拿来为一种以黏菌为食的甲虫Agathidium bushi命名。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

Myxomycetes ; spore ; nutrient decoction ; pH ; hanging drop culture

黏菌；孢子；营养稀煎汁； pH ；悬滴培养

In order to clarify the genetic mechanism of the adhesion,an in vitro adhesion experiment was carried out for three variants of E.coli F4in 366 piglets of three pig breeds.The results showed that there existed significant differences(P.001)in the adhesion percentage among the three breeds.Most SB piglets were nonadhesive for all the three variants,whereas most LR piglets were adhesive.Within each breed except for LR,the proportions of the three F4 variants adhering to the brush borders differed significantly.

为了研究黏附的遗传机制，本实验利用大白、长白、松辽黑猪的小肠刷状缘细胞与F4ab、F4ac、F4ad进行离体黏附实验，结果发现3品种猪之间黏附情况存在显著差别(P《0.01)，松辽黑猪以非黏附型为主，而长白猪中黏附型比例较高，在同一品种猪内，3种菌株的黏附比例在松辽黑猪内和大白猪内有极显著差异，但在长白猪中无显著差异。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The cells in question are a specially grown type of" slime mould" that naturally shies away from light.

本文所谈到的细胞，是一种天生迴避光线的黏菌的成熟型态。

Hanging drop cultures were used to study the process of spore germination of 8 species from 2 orders in some nutrient decoctions and at various pHranges.

利用悬滴培养法对2个目8种黏菌孢子在各种营养稀煎汁和不同pH条件下的萌发情况进行了研究。

In vitro time-killing studies, the combination of PEI-amphotericin B and PEI-fluconazole enhanced antifungal activity, but there was an antagonist for the combination of PEI-polymyxin B.

在体外致死研究实验中可以看到，PEI-两性霉素B和PEI-氟康唑结合物可以提高抑菌作用，但PEI-多黏菌素B结合物却拮抗抑菌作用。

Objective: To explore the roles of vascular endothelial growth factor in hypoxia pulmonary hypertension and effect of Polymyxin B.

目的：探讨血管内皮生长因子在低氧性肺动脉高压形成中的意义及多黏菌素B对其的影响。

The clinical application effects of levofloxacin and polymyxin E needed to be proved with experiments.

结论］左氧氟沙星与多黏菌素配合的临床应用效果有待于进一步试验证明。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。