黄酮

Keywords HPD-600macroreticular resin;total flavonoids of Crataegus leaves;separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

HPD-600macroreticular resin ; total flavonoids of Crataegus leaves ; separation and purification

HPD-600大孔树脂；山楂叶总黄酮；分离纯化；正交实验

Objective: To investigate the adjust fat possible mechanism of Flavone mixture of crataegus leaves through observing the effect on proliferation, differentiation and the excrete of Leptin, PMI-1 of 3T3-L1 preadipocyte.

目的：观察山楂叶总黄酮对前脂肪细胞3T3-L1增殖、分化和分泌Leptin、PAI-1的影响，探讨其调脂作用的可能机制。

The HPLC system column, mobile phase of CH3CN-3%CH3COOH (11∶89) mixture, with detection wave at 270 nm, was used to determine the content of vitexin in Leaf of Crataegus pinnatifida Bge.

应用反相高效液相色谱法测定了山楂叶总黄酮中牡荆素的含量。C18柱作为色谱柱，乙腈-3%醋酸水溶液(11∶89)为流动相，检测波长270 nm，按外标法定量。

Objective: Cynomorium contains a variety of chemical composition, flavonoids are the main features of one of the ingredients.

目的：锁阳中含有多种化学成分，黄酮类化合物是其主要功能性成分之一。

The main experiment on supercritical CO2 extraction technology Cynomorium flavonoids process to explore and identify better conditions for the extraction.

本实验主要对超临界CO2流体技术提取锁阳黄酮的工艺条件进行探讨，以及确定较好的提取条件。

Methods: The single factor experiments, systematically study the extraction of temperature, pressure extraction, the extraction time, an attachment ratio (Cosolvent velocity: carbon dioxide flow) and samples of the particles Cynomorium rate of flavonoids extracted.

通过单因素实验，比较系统地研究了萃取温度、萃取压力、萃取时间、夹带比（夹带剂流速：二氧化碳流速）及样品颗粒度对锁阳黄酮提取率的影响。

Spectrophotometry was used to determine the flavanone of Cyperus rotundus .

结果测得样品中总黄酮的含量C=0.186 4 mg/ml，回收率为102.7%，其纯度和产率均较高。

ResultBy this method the content of the total flavanone of Cyperus rotundus was C=0.186 4 mg/ml and the rate of recovery was 102.7%.

结论该方法采用全物理过程，无任何污染，是提取香附黄酮类物质的有效途径。

MethodsThe model of cerebral ischemia and anoxia in mice was established by decollation and ligating common bicarotid artery the effect of BVF on the survival time of the mice,and the time of catching their breath after mice decollated,and mouse brain capillary vessel permeability were observed.

方法采用小鼠断头、结扎双侧颈总动脉等脑缺血缺氧模型，观察苦菜总黄酮对脑缺血缺氧小鼠存活时间、断头小鼠喘息时间及脑毛细血管通透的影响

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。