黄酮

Objective:To extract and determine total flavonoids and polysaccharides in Ephedra sinica.

目的：从麻黄中提取总黄酮和多糖，并测定其含量。

The contents of nine trace elements and constant elements in Erigeron BreviscapusHand.

目的研究中药灯盏花中总黄酮及微量元素的含量，为治疗心脑血管疾病的机理提供科学的依据。

Objective To observe contents and effect of total flavonoids of acanthopanax senticosus extract on erythrocyte membrane fluidity.

目的 观察刺五加提取物中总黄酮的含量及其对红细胞膜流动性的影响。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The proposed method surpassed other chromatographic alternatives in terms of limit of detection at least 10~3 folds with good reproducebility. Chapter 4 A capillary zone electrophoresis with indirect LIF detection method is developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside).

第四章建立了一种毛细管区带电泳—间接激光诱导荧光检测法同时测定6种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的新方法，扩大了LIF检测的应用范围。

BACKGROUND: Cristata L flavonoid is a kind of plant estrin, which possesses multiple physiological function, has no toxicity and adverse effect, and is effective in treating and preventing osteoporosis.

背景：鸡冠花黄酮类化合物是一种植物性雌激素，具有多种生理功能，且无毒副作用，对骨质疏松的治疗和预防都具有良好干预效果。

ObjectiveTo determine total flavonoid content in Eugenia caryophyllata Thunb. by VIS and UV Spectrophotometry.

目的研究可见光及紫外分光光度法测定丁香总黄酮含量的方法。

ObjectiveTo optimize the extraction condition for Eupatorium adenophorum Spreng by response surface methodology.

目的利用响应曲面法研究从紫茎泽兰中提取黄酮的最佳工艺条件。

This paper summarizes research adcance of flavonoids in medical value and methods of exaction and separation from different kinds of plants.

对黄酮类化合物的药用价值、提取工艺、分离方法等方面进行综述。

Based on single-factor experiments, orthogonal experiments were performed to optimize the extractive conditions.

在单因素实验的基础上，设计了的正交实验来确定溶剂提取大豆异黄酮的最佳工艺条件。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。