黄酮

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

On the basis of the single-factor experiment, the quaternionic quadratic rotational combination was used to optimize the extraction process of total relative flavone that came from fermentation fluid of citrus, and we had used the response surface methodology to study the best horizontal extent of solid-to-liquid ratio, extraction time, alcohol density, the microwave power which affected the rate of total flavone relative extraction.

在单因素试验的基础上，采用四元二次通用旋转组合试验优化柑橘皮渣发酵液总黄酮的提取工艺，用曲面响应法对影响总黄酮得率的料液比，提取时间，乙醇浓度，微波功率的最佳水平范围进行研究。

The extraction process and content examination were studied with the methods of orthogonal test and spectrophotometry compared with Rutin; then the total flavonoids from Ramulus Mori were extracted with the methods of phytochemistry and examined.

以芦丁为对照品，运用正交设计试验及分光光度法，对桑枝总黄酮(the total flavonoids from Ramulus Mori，RMTF)的提取工艺、含量测定进行了探索；然后，采用植物化学方法提取并定性检测桑枝总黄酮，进而开展动物降血糖药理试验。

Results:The cell aggregations had differences in shape as well as size, flavone content, enzyme activities, growth speed and so forth. The flavone content of the smallest cell aggregation was approximately twice compared to the larger ones, and its malonaldehyde content, the phenylalanine ammonialyase and peroxide enzyme activities and recultivate curve presented the same change tendency.

结果：不同的细胞聚集体在形态大小、黄酮含量、酶的活性、生长速度等方面均存在着差异，其中颗粒最小的细胞聚集体黄酮含量约为颗粒较大的细胞聚集体的2倍，并且其丙二醛含量、苯丙氨酸解氨酶及过氧化物酶的活性及再培养曲线均具有相同的变化趋势。

Resuit Temperature had the most effect on the extraction of flavone.

结果结果表明，温度对黄酮提取影响最大，固液比1：60，在90℃下，每次1h，重复提取3次，黄酮提取率最高。

Qinghao acid, Qinghao B, Scopoletin, Casticin and Chrysosplenol D had some antimalarial activities on P. berghei. Even when the dosage was up to 500 mg/kg/d, the maximal inhibition rate was only 59%, which is less active than QHS. Therapeutic effect of different ingredients of A.

青蒿酸、青蒿乙素、东莨菪内酯、青蒿黄酮1、青蒿黄酮2 5种成份对鼠疟均有不同程度的抑制作用，但剂量高达500mg/kg/d时，抑制率最高也仅达到59%，远低于QHS的疗效。

Objective To study the effects of factors like solvent dosage,extraction time and extraction times on the production extraction of flavonoids from Scutellaria baicalensis .

目的探讨黄芩中总黄酮提取工艺中的提取溶剂用量、提取时间、提取次数对黄芩中总黄酮得率的影响。

This paper compared two kinds of rose families sorbus of different effective spots of total flavonids content. We get a better process of total flavonoids abstraction from the branch of Sorbus tianschanica,.Under the best process of total flavnoids ,two flavnoids were isolated from them. Preliminary affirmed two kinds of compounds belong to flavanone, two kinds of structure just substituent had a dissimilarity separately. This thesis was a part of a national natural science fund to subsidize.

除此，本论文还对新疆天山花楸的枝干总黄酮的提取工艺进行了研究，得到了最佳提取工艺，在最佳提取工艺的条件下对新疆天山花楸的枝干进行了系统的提取和硅胶柱层析，得到两种化合物，初步认定两种化合物属于二氢黄酮类，两种化合物的结构相似，只是个别取代基有所不同，本论文是国家自然科学基金资助项目。

In addition, rutin has many other functions, such as anti-inflammatory, antiallergic, diuretic, spasmolytic, reducing blood-fat, cardiotonic, protecting ulcer surfaces, etc. Therefore it has been used in clinical treatment.

芦丁，3，3′，4′，5，7，—五羟基黄酮-3-芸香糖苷，又名芸香苷，是一种从植物中提取得黄酮类化合物，无论在什么条件下，都具有多方面的生理活性和电化学活性。

METHODSUsing orthogonal design, total flavonoids in extract was as evaluation index, and ethanol concentration, extraction time, ratio of material to solvent and extration frequency were as inspecting factors to screened the optimum extraction technique, and total flavonoids content in extract was detected by spectrophotography.

方法］采用正交设计法以提取物中总黄酮含量为评价指标，以乙醇浓度、提取时间、固液比及提取次数为考察因素，筛选翻白草的最佳提取工艺。应用分光光度仪测定提取物中总黄酮的含量。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。