黄连素

There are some reports about the application of Berberine in medicine and agriculture in recent years.

对黄连素在医学和农学领域的研究状况加以综述，并对黄连素潜在的应用价值和良好的发展前景进行展望。

Toxicity and repellent activity of several plant-based compounds on Blattella germanica were studied, results showed that rotenone, toosendanin and azadirachtin had stomach toxic effect on Blattella germanica, rotenone showed high stomach toxicity and all insects died after 8 hours, matrine also has deformative effect on Blattella germanica , matrine and toosendanin can affect oviposition of Blattella germanica; repellent activity of these botanicals was further studied, results showed that bering hydrochloridum, azadirachtin and sparteinehave had repellent activity against Blattella germanica, observation also showed that rotenone and bering hydrochloridum had high contact toxicity against Blattella germanica, the time of all insects knocked down were 8 and 48 hours.

选取了7种植物源化合物，测定了它们对德国小蠊的毒性和驱避性，结果表明，鱼藤酮、川楝素和印楝素对德国小蠊具有不同程度的胃毒作用，其中鱼藤酮乙酸乙酯溶液作用较强，8小时死亡率已达100％，川楝素和印楝素对德国小蠊也表现出一定的毒杀作用，观察还发现印楝素和苦参碱对德国小蠊的发育有致畸作用，苦参碱和川楝素影响其卵鞘形成；进一步对这几种化合物的驱避性进行研究，结果表明盐酸黄连素、鱼藤酮乳油和印楝素乳油对德国小蠊有不同程度的驱避作用，其驱避率分别为96.7％、51.3％和50.1％，另外观察到鱼藤酮乙酸乙酯溶液和盐酸黄连素对德国小蠊具有较强的触杀活性，供试虫全部被击倒的时间分别为8小时和48小时。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or P .01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or P .01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05， P 。01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05， P 。01）；4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or 0.01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or 0.01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05或0.01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05或0.01）；4种单体对过氧化氢损伤无显著的作用。

It could depress the side effect side effect of berberine Through culture ang detrain the Bifidobactirium that proof berberine ,and take medicine go with berberine .

通过体外培养分离出耐黄连素的双歧杆菌，配合黄连素服用可降低黄连素的副作用。

The model of thyroid hormone-induced diarrheic rats was prepared and interfered by low, medium and high dose of Asi-antidiarrheal capsule and berberine. Immunohistochemistry was used to detect the population, ratio of degranulation, area, perimeter, equivalent diameter and cyclo-dimension of the MC in duodenum mucosa of control group, model group, berberine group and Asi-antidiarrheal capsule group.

运用阿是止泻胶囊低、中、高剂量及黄连素对其干预，采用免疫组化技术测定对照组、模型组、黄连素组、阿是止泻胶囊低、中、高剂量组大鼠十二指肠黏膜MC数量、脱颗粒比率、面积、周长、等效直径及圆形度。

Berberine is mainly used to treat major used in enteric bacterial infection,recently,researches showed that Berberine can not only be used to treat cardiovascular disease,endocrine disease,nutritional and metabolic disease,central nervous disease,tumors,but also can improve immune function and anti-graft rejective reaction of skin besides its antibacterial action.

黄连素在临床上主要用于治疗肠道细菌感染，但大量研究和临床应用表明，黄连素除了有抗菌作用外，还可用于治疗心血管疾病、内分泌、营养及代谢疾病、中枢神经系统疾病、肿瘤等疾病，同时具有增强免疫机能及抗皮肤移植排斥反应等作用。

Put the detrained Bifidobactirium in different consistency berberine-culture medium and keep on screen Bifidobactirium that proof berberine.

将分离出来的双歧杆菌在不同浓度的黄连素培养基上培养，不断筛选获得抗黄连素的双歧杆菌。

The oxidative modification of low-density lipoprotein is thought to have a central role in the pathogenesis of atherogenesis. Berberine, a natural constituent of plants of the genera Coptis and Berberis, has several anti-inflammation and anticancer biological effects.

黄连素 是黄连中主要的天然化合物，目前已知具有抗发炎(anti-inflammation)和抗肿瘤等生物活性，然而对於黄连素是否会抑制粥状动脉硬化形成及其详细的作用机制到目前为止并不清楚。

The average peak time values of berberine nanoemulsion (4.205 h) enhance 2.826 h,3.088 h than tablet(1.379 h) and capsule(1.117 h).The average highest plasma concentration of its (113.699μg/L)is 1.820,1.894 times of ablet (62.466μg/L)and capsule(60.021μg/L), The relative bioavailability is339% and 332% that of tablet and capsule.

黄连素纳米乳的平均达峰时间Tmax(4.205 h)比黄连素片剂(1.379 h)和黄连素胶囊(1.117 h)延长2.826 h和3.088 h；其理论的平均最高血药浓度Cmax(113.699μg/L)是黄连素片剂(62.466μg/L)和黄连素胶囊(60.021μg/L)的1.820倍和1.894倍；其相对生物利用度是黄连素片剂和黄连素胶囊的339%和332%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。