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黄萎病

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The disease control effect of Verticillium wilt of cotton was 15% to 30% higher than that of seed soaking with the carbendazim, with the yield increase rate of 10% to 15%; The powdery mildew of wheat, strawberry, and cucurbits, at their early stage , were sprayed with AQ 0116 every five to seven days, three to five times totally.

该水剂通过浸种结合两次叶面喷雾的方式防治棉花黄萎病,防效比多菌灵浸种处理高15%~ 30%,使棉花增产10%~ 15%;该水剂在小麦,草莓和瓜类白粉病发病初期喷雾,5~7d一次,连续施用3~5次,对小麦,草莓和瓜类白粉病的防效分别为76.3%,90.0%,97.0%。

The fermentation conditions of the antagonistic bacteria 12-47 to cotton wilt were studied by using the single-factor method. The main factors affecting fermentation conditions include C and N sources, inorganic salts, pH, temperature, rotation speed.

本实验采用单因素实验的方法在摇床培养的基础上对影响棉花黄萎病拮抗细菌12-47菌株产生拮抗物质的营养因素进行了考察,其中包括发酵培养基的C源、N源、无机盐等。

Bacillus subtilis NCD-2 was an effective bacterial strain to control verticillium wilt of cotton,and could produce antibiotic substances to inhibit the gowth of Verticillium dahliae,the pathogen causing this disease.

枯草芽孢杆菌NCD-2菌株是一株有效防治棉花黄萎病的细菌,它通过产生抑菌物质达到对大丽轮枝菌的抑制作用。

Cotton ; Verticillium wilt ; resistance mechanism phytoalexin

棉花;黄萎病;抗性机制;植保素

After cotton plants are infected by V. dahiiea, the formation of gums and tyloses and the eventual sealing-off of the infected vessels are occured in plants or phytoalexin are producted. Physical restriction wilt or che...

棉株遭受黄萎病菌侵染后,以其内部形成一些胶状体、侵填体等物理障碍或产生一些植保素等生化障碍或两者同时发生的反应形式来表现抗性。

The result of study indicated: the isolate that was obtained in the test was pathogen causing tomato Verticillium wilt, and it was Verticillium dahiea kleb .

根据供试菌株在鉴别寄主上的反应,可以确定本试验分离并利用的番茄黄萎病菌为生理小种1(Vercitillium dahiea race 1)。

At the same time, the study found an easy and simple resistant identification method in seedling stage and carried on resistant identification of Verticillium dahiea wilt to tomato germplasm resources that are kept by our lab.

生物学特性研究表明,适于番茄黄萎病菌生长的培养基为PSA培养基,低量蔗糖酵母培养基,马铃薯胡萝卜培养基及蔡氏培养基;在马铃薯胡萝卜培养基上产孢效果最理想。

On soil fungi, Fusarium root shell mold, Rhizoctonia, Pythium, Miao-rot fungus, Corticiumpathogens have a significant effect on the damping-off,diseases, damping-off, Fusarium wilt, Verticillium, Sclerotinia, Colletotrichum, disease, dry rot, scab, Sclerotinia rot, seedling blight, stem blight, leaf blight, steep root, even the special effects for the continuous cropping obstacles.

对土壤真菌、镰刀菌、根壳菌、丝核菌、腐霉菌、苗腐菌、伏革菌等病原菌都有显著的防治效对立枯病、烂秧病、猝倒病、枯萎病、黄萎病、菌核病、炭疽病、疫病、干腐病、黑星病、菌核软腐病、苗枯病、茎枯病、叶枯病、沤根、连作重茬障碍有特效。

Extension of the phosphorus depletion zone in VA-mycorrhizal white clover in a calcareous soil.

关于VA菌根菌与棉花黄萎病菌存在侵染中的竞争作用。

Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选,在 PAP基因的 3'端融入了绿色荧光蛋白GFP)基因,然后将融合基因克隆在植物表达载体PBI 121上,再进行遗传转化,得转基因棉花种子5000余粒,将种子播种长到于叶展开时,先在黑暗中用紫外灯照射,查找表现绿色荧光的幼苗,然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交,最后发现有8株棉花表现阳性反应,说明PAP基因的确己经转到了棉花的基因组中,其棉花黄萎病的抗性鉴定正在进行之中。

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