黄皮

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术，观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响，结果证实了促孕液治疗卵巢静止、持久黄体的机理：(1)微弱的雌激素样作用，能刺激卵泡发育，促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化；(2)提高子宫内膜碱性磷酸酶的活性；(3)改善微循环；(4)促进子宫运动。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The proposed method surpassed other chromatographic alternatives in terms of limit of detection at least 10~3 folds with good reproducebility. Chapter 4 A capillary zone electrophoresis with indirect LIF detection method is developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside).

第四章建立了一种毛细管区带电泳—间接激光诱导荧光检测法同时测定6种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的新方法，扩大了LIF检测的应用范围。

Phenolics detected in C. incanus L. bee pollen belong to flavonol, flavanols (quercetin, kaempferol, galangin, and isorhamnetin), flavones and phenylpropanoids.

酚类物质检测长梅花湖蜂花粉属于黄酮，黄烷醇（槲皮素，山奈酚，高良姜素和异鼠），黄酮和苯丙。

Because the accumulation of flavonoids was the foundation of producing mottling on seed coat, the composition and changes of content of flavonoids were related to resistance level to seed coat mottling in mature seed.

类黄酮在种皮上的积累为形成斑驳构成了物质基础。成熟种子种皮中类黄酮配基的组成和含量变化与抗种粒斑驳的抗性相关。

After being purified by the B-8 resin, the contents of the total flavonoids and quercitrin were raised 2.48 times and 3.29 times more than those before purification respectively.

总黄酮与槲皮苷的保留率达70%、95%以上，峰1~3的保留率达90%以上，纯化后总黄酮及槲皮苷的含量分别提高了2.48倍和3.29倍。

Methods Spectrophotometric method was used to detect the total flavonoids, and HPLC was used to determine quercitrin and analyze the characteristic peaks of Fingerprint.

以总黄酮、槲皮苷含量结合指纹图谱主要特征峰面积保留率为考察指标；采用可见分光光度法测定总黄酮含量，HPLC法测定槲皮苷含量，HPLC法分析指纹图谱主要特征峰面积。

Advances in studies on formation and inheritance of seed capsule color,and molecular marker and breeding for yellow seed capsule in rapeseed were reviewed.

综述了油菜种皮颜色的形成、种皮色泽的遗传、黄籽性状的分子标记和黄籽甘蓝型油菜选育等方面的研究进展。

METHODS The contents of synephrine,neohesperidin,hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3(4.6 mm×250 mm,5 μm) column, respectively.

方法采用高效液相色谱法，以InertsilODS-3(4.6mm×250mm，5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新橙皮苷、橙皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。

Lithocarpus polystachyus Rehd with rich valuable flavonoid and phloridzin, is an abundant plant resource in our country, located mainly as wild plants in the mountain area in southern China, and its rich resource of flavonoid and phloridzin has not been developed.

多穗柯是我国特有的一种丰富植物资源，富含价值很高的黄酮类及其根皮苷，目前主要以野生状态分布于江南山区，其丰富的黄酮类及其根皮苷资源尚处于完全未被开发利用状态。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。