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Pepper ; cucumber mosaic virus ; CP gene ; 2b gene ; sequencing

辣椒;黄瓜花叶病毒; CP基因; 2b基因;序列分析

One satellite RNA Was found associated with cucumber mosaic virusfrom banana.

摘要1。从患香蕉花叶心腐病香蕉病叶分离的黄瓜花叶病毒中,发现一种新的卫星RNA。

Tabacoo mosaic virus ; Cucumber mosaic virus ; Potato X virus ; RT-PCR

烟草花叶病毒;黄瓜花叶病毒;马铃薯X病毒; RT-PCR

Cucumber green mottle mosaic virus ; reference material

黄瓜绿斑驳花叶病毒;参照物质

A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法,扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因,将其克隆到pKEN上,构建了该融合基因的原核表达载体pKENG-M。

The origin analysis on the source of hosts and terrains was carried out on the base of homology tree and phylogenetic tree founded in 19 isolates on their CP gene nucleotide sequences. The result showed that the gene types and clusters of Cucumber green mottle mosaic virus were not obviously related to the two factors.

对包括该分离物在内的19个CP基因核苷酸序列进行同源性比较和系统进化树构建,并结合寄主来源及地域来源进行LHP的起源分析,结果表明黄瓜绿斑驳花叶病毒不同分离物的序列分群和进化关系与上述两个因素无明显关系。

Cucumber mosaic virus;nucleotide diversity;selection;reassortant;variability

黄瓜花叶病毒;核酸多样性;选择;重排;变异

For the soybean germplasms, 76(25.33%), 41(13.67%) and 14 (4.67%) were positive to Soybean mosaic virus, Cucumber mosaic virus, and Alfalfa mosaic virus respectively.

结果表明:在大豆种质中检测出大豆花叶病毒、黄瓜花叶病毒、苜蓿花叶病毒三种病毒,阳性检出率分别为25.33%(76份)、13.67%(41份)和4.67%(14份)。

Based on the genome conservative sequence of tabacoo mosaic virus, cucumber mosaic virus and potato X virus, special primers were designed respectively. With the RT-PCR, the target fragments about 313,326 and 392 bp were ampilfied separately from the infected sample, but not health sample.

设计烟草花叶病毒、黄瓜花叶病毒和马铃薯X病毒的特异性引物,RT-PCR检测,从感病叶片中分别扩增出313,326,392 bp的目的片断,而健康叶片中无此扩增带。

Cucumber mosaic virus was detected from infected Piper nigrum Linn. in hainan with the indirect enzyme-linked immunosorbent assay.

通过间接酶联免疫法,从海南罹病胡椒植株中检测到黄瓜花叶病毒(Cucumber mosaic virus,CMV)。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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