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黄嘌呤

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Methods 2BS cell lines were treated with Tuber Fleeceflower Root serum. The activity of c-myc mRNA was detected by semi-quantitive RT-PCR. A TRAP-ELISA method was used to measure the telomerase activity.

采用含何首乌血清对2BS细胞株进行处理,用黄嘌呤氧化酶法检测SOD活性,RT-PCR检测c-myc mRNA表达,TRAP-ELISA检测端粒酶活性。

After it gets pasteurized all the valuable enzymes in milk are destroyed (lactase for the assimilation of lactose; galactase for the assimilation of galactose; phosphatase for the assimilation of calcium).

但是当我们将脂肪打散成很小的例子时,许多人相信同时会产生一种化学物质称为—黄嘌呤氧化酶这是均质化的牛奶被认为容易造成癌症的原因。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪,通过机械分割和组织消化法获取脂肪基质细胞,在内皮细胞培养液中培养7d后,经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征,再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤,1μmol/L地塞米松,10μmol/L胰岛素,200μmol/L吲哚美星)定向诱导7d,形态学观察和油红O特殊染色鉴定诱导后的细胞。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

The ARF model of rabbit was copied by 1% HgCl 2,the malondialdehyde,superoside dismutasecontent in serum and renal homogenate were determined by modificatory TBA microdetermination,xanthinoxidanse method.

以 1%HgCl2 复制家兔ARF模型,分别应用改良TBA微量法、黄嘌呤氧化酶法测定血清及肾组织匀浆的丙二醛水平、超氧化物歧化酶活性的变化。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估:手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量;应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平;以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜;以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

The rats of model group,acupointⅠgroup, acupointⅡgroup were treated with passive smoking.The rats of acupointⅠgroup,acupointⅡgroup were treated with placing Chinese medicine at the point of feishu,pishu for 5 hours or 10 hours after depilation.

治疗结束后观察支气管组织病理特征及细胞浸润情况,并对其进行气道炎症病理评分;酶联免疫吸附法测定被动吸烟大鼠肺泡灌洗液中α_1-抗胰蛋白酶、TNF-α、IL-8含量;黄嘌呤氧化酶法测定被动吸烟大鼠肺泡灌洗液SOD的活性、TBA法测定MDA的含量。

We studied the radical scavenge enzyme and radical metabolite lipid peroxide in vesical carcinoma tissue and the paracancerous tissue of 32 patients by Xantine Oxidase method and propiolaldehyde method.

应用黄嘌呤氧化酶法和丙二醛法对32例膀胱癌及其癌旁组织中自由基清除酶超氧化物歧化酶活性和自由基代谢产物过氧化脂质含量测定。

The results are in good agreement with available experimental results. The entropy effect on the Gibbs free energies of 6-thioxanthine base is very small and there is little significance for the tautomeric equilibria of the base.

计算结果同已有实验结果一致。6-硫代黄嘌呤异构化的熵效应小,对互变异构平衡几乎没有显著的影响,而焓变对互变异构产生了主要的影响。

Methods C3b sensitized yeast cells and C3b unsensitized yeast cells were used to detect C3b receptors and immune complex on red cells,method of xanthine oxidase was applied to detect SOD activity in red cells.

实验利用低碘饲料和碘化钾去离子水复制了不同碘营养状态的大鼠,采用C3b致敏和未致敏的酵母菌及黄嘌呤氧化酶法分别检测红细胞膜上的C3b受体、免疫复合物及红细胞内SOD的活性。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?