黄

Compared with solvent extraction, the extraction time of microwave extraction was reduced from 20 h to 450 s, the extraction rate was increased from 79.86% to 92.23%, and that AB-8 was used to extract Chimonanthus praecox Link. flavonoids were very effective and low cost.

与溶剂浸提法相比，采用微波法提取腊梅花总黄酮提取时间由20 h缩短为450 s，提取率从79.86%提高到92.23%，而且AB-8分离腊梅花总黄酮分离效果明显，成本低。

It changed to be strong reflection after degeneration.2.On the growth of follicle,follicular cell produced prominency which keeps touch with oocyte rightly.

在发情周期内，有大小不一的发育卵泡和黄体；卵泡显示为无回声的团状黑影；黄体为均匀的深灰色细小颗粒组成，退化后成为强反射。2。

Results There were 5 species of indoor free flea in Hepu County and Pulex irritans was dominant, accounted for 93.40%.

结果合浦县黄胸鼠体共有6种寄生蚤，年平均鼠密度为4.09％，平均染蚤率为43.13％，黄胸鼠体印鼠客蚤指数为1.0982。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

On the basis of the single-factor experiment, the quaternionic quadratic rotational combination was used to optimize the extraction process of total relative flavone that came from fermentation fluid of citrus, and we had used the response surface methodology to study the best horizontal extent of solid-to-liquid ratio, extraction time, alcohol density, the microwave power which affected the rate of total flavone relative extraction.

在单因素试验的基础上，采用四元二次通用旋转组合试验优化柑橘皮渣发酵液总黄酮的提取工艺，用曲面响应法对影响总黄酮得率的料液比，提取时间，乙醇浓度，微波功率的最佳水平范围进行研究。

The extraction process and content examination were studied with the methods of orthogonal test and spectrophotometry compared with Rutin; then the total flavonoids from Ramulus Mori were extracted with the methods of phytochemistry and examined.

以芦丁为对照品，运用正交设计试验及分光光度法，对桑枝总黄酮(the total flavonoids from Ramulus Mori，RMTF)的提取工艺、含量测定进行了探索；然后，采用植物化学方法提取并定性检测桑枝总黄酮，进而开展动物降血糖药理试验。

Objective To determine the susceptibility of Rattus rattoides to bromadiolone and provide base data for R.rattoides resistance to bromadiolone.

目的 测定黄毛鼠对第二代抗凝血灭鼠剂溴敌隆的敏感性，为黄毛鼠的抗药性检测提供敏感种群本底资料。

Results:The cell aggregations had differences in shape as well as size, flavone content, enzyme activities, growth speed and so forth. The flavone content of the smallest cell aggregation was approximately twice compared to the larger ones, and its malonaldehyde content, the phenylalanine ammonialyase and peroxide enzyme activities and recultivate curve presented the same change tendency.

结果：不同的细胞聚集体在形态大小、黄酮含量、酶的活性、生长速度等方面均存在着差异，其中颗粒最小的细胞聚集体黄酮含量约为颗粒较大的细胞聚集体的2倍，并且其丙二醛含量、苯丙氨酸解氨酶及过氧化物酶的活性及再培养曲线均具有相同的变化趋势。

At last, he introduced his book" why time don't regorge " which was about experiences of Huang.

最后黄教授重点介绍了他的书《为什么时光不能倒流》，这本书讲述的是黄教授的过去的种种经历，对生活中有迷茫或者困惑的同学应该有所帮助。

Resuit Temperature had the most effect on the extraction of flavone.

结果结果表明，温度对黄酮提取影响最大，固液比1：60，在90℃下，每次1h，重复提取3次，黄酮提取率最高。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。