麻黄碱

In chapter two, we demonstrated the separation of five ephedrine alkaloid isomers in NACE.

论文第二章中，首次建立利用NACE分离五种麻黄碱类生物碱异构体的方法。

These drugs include atropine, belladonna, cascara , castor oil, cocaine, curare, ephedrine, morphine, opium, and senna.

这些药物有：阿托品，颠茄，波西鼠李皮，海狸香油，可卡因，箭毒，麻黄碱，吗啡，鸦片和番泻叶。

Methods Chinese ephedra, liquorice and honeysuckle in apricot kernel cough mixture were qualitative examined by thin-layered chromatography, and the content of ephedrine hydrochloride was measured by high performance liquid chromatography.

采用薄层色谱法对麻杏止咳合剂中麻黄、甘草、金银花进行定性鉴别，采用高效液相色谱法测定麻杏止咳合剂中盐酸麻黄碱的含量。

8 Ephedrine is metabolized to norephedrine which is responsible for the central nervous system stimulating effects of the drug.

，麻黄碱代谢，以去甲麻黄碱是负责对中枢神经系统的刺激作用的药物。

Objective To develop a method for determination of Ephedrine Hydrochloride in Ephedrine Hydrochloride nasal drops by HPLC.

目的 建立用高效液相色谱法测定盐酸麻黄碱滴鼻液中盐酸麻黄碱含量的方法。

LC separation of ephedrine and methylephedrine was established, and it improved anti-interference ability and detection limit.

本研究建立麻黄碱及甲基麻黄碱的液相分离条件，提高了检测的抗干扰能力和检测限。

Assuming phenylephrine100 g to be approximately equipotent to ephedrine 8 mg,the groups contained the proportional potency equivalent of100%, 75%, 50%, 25% or 0% of phenylephrine and 0%, 25%, 50%,75% or 100%, respectively, of ephedrine.

假设100ug 去氧肾上腺素约相当于8mg 麻黄碱，那么五组混合物中就分别含有去氧肾上腺素等效剂量100%、75%、50%、25%或0%及麻黄碱0%、25%、50%、75%或100%。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Results: Under the experimental conditions adopted, the baseline separation of ephedrin and ephedrine levorotatory was obtained.

结果：麻黄碱及伪麻黄碱对映体得到良好分离，并满足进行左旋麻黄碱中对映异构体杂质检查所需要的条件。

Regard hydrochloric acid of Mahuang as the index, adopt HPLC to determine the content of the hydrochloric acid of Mahuang , whether through is it test excellent to elect the best technology to hand in.

以盐酸麻黄碱为指标，采用高效液相法测定盐酸麻黄碱的含量，通过正交试验优选出最佳工艺：取原药材饮片，加10倍量的60%乙醇，回流提取三次，每次105min的制备工艺最佳。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。