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麻醉乙醚

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The rats were anesthetized with diethyl ether continuously.

大鼠持续乙醚麻醉下,分别在距离肛门约3.5cm用针长8cm的1ml注射器

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是:1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气,取小部分冷冻保存;2、在乙醚麻醉下,快速断头并在冷冻液里取出包含海马的大脑部位,修整出所需海马区;3、将海马组织固定于振动切片机上切出300μm厚的海马脑片,用广口吸管转移至预孵育皿培养一小时,后再转移到记录用孵育槽内;4、拉制好玻璃微电极,并充以3mol/L的NaCl;5、调试好记录仪器,将脑片在孵育槽内培养2小时后进行实验;6、为了保证实验结果的准确性,每一块脑薄片只进行一个浓度的药品实验。

The urine of experimental animals was collected using metabolic cages once a week for 12h. The mice were sacrificed under ether narcotization 14 days after the injection of NTS. Sera and kidney tissues were obtained at sacrifice.

小鼠每周一次使用特制新陈代谢器收集12小时尿液,并在注射肾毒血清二周后用乙醚麻醉,全数牺牲以采集血清及肾脏组织。

On the last dayof the 8th week of the training period, the exhausted time of both exercise exhaust group and drug-taken exhaust group should be recorded. Then, all of the objects are weighted, hocused by aether and taken the materials from some tissues.

训练至第八周最后1天,除对照力竭组和加药力竭组在力竭运动后记录力竭时间、称重外,其余三组即安静对照组、运动对照组和运动加药组大鼠称重后,用乙醚麻醉、取材。

In this study we sought to determine whether exposure to theanesthetics diethyl ether and isoflurane influences the activityof hepatic cytochrome P450 2E1 and P450 reductases in the rat.

通过这项研究,我们试图去确定用乙醚或异氟醚麻醉是否影响肝脏细胞色素酶 P450 2E1和 P450还原酶的活性。

Methods :90 SD rats were randomly devided into control group (1 month, 2 months, 3 months) and model group (1 month, 2months, 3 months), made rat models by pouring Lipopolysacchride into trachea and putting rats into smoking environment, and anaesthetised with ether, striped the spleen and thymus, and weighted.

将90只SD大鼠随机分为对照l个月、2个月、3个月组和模型l个月、2个月、3个月组,采用气管内注入脂多糖和烟熏双因素复合制作肺气肿肺气虚证大鼠模型,乙醚吸入法麻醉后剥离胸腺和脾脏,电子天平称重。

Rats were fed a purified diet for 6 wk before anesthesia with1 of 3 anesthetics: carbon dioxide, diethyl ether, or isoflurane.

大鼠在用纯净的饲料喂养6周后,分别用二氧化碳、乙醚、异氟醚麻醉。

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