麝香草酚蓝

After studying the color reaction of thymol blue with berberine, we find that in the buffer solution of NH3-NH4Cl, whose pH is 10.5, the color reaction is best by repeated experiment and verification.

本文对麝香草酚蓝与盐酸小蘖碱的显色反应进行了研究，发现在pH=10.5的NH3-NH4CL缓冲溶液中显色效果最好，此反应只要在室温下就能显色完全，且稳定性好。

The thesis applies the principle that organism and organic reagent can visualize in some condition and produce color association polymer. We have tried several organic reagents in order to build color reaction for berberine; at last, we find that thymol blue can react with berberine with good results.

Spectrophotometric Determination of Berberine using Thymol Blue-化学工程与工艺-利用有机化合物与有机试剂在一定的条件下能显色生成有色缔合物的原理，尝试了铬兰黑R、百里香酚蓝、甲酚红等多种有机试剂与盐酸小蘖碱的显色反应，结果发现麝香草酚蓝与盐酸小蘖碱的显色反应较好。

METHODS Acidic dye colorimetry with bromothymol blue as ion pair reagent was employed to determine the alkaloids in samples.

用溴麝香草酚蓝同川芎生物碱形成稳定的有色离子对酸性染料比色法测定总生物碱的含量。

The proposed method involves forming coloured ion-pair from ephedrine hydrochloride and bromothymol blue at pH 6.5 extracting the ion -pair thus into chloroform and measuring the absorbance chloroform solution at 414 nm.

根据盐酸麻黄碱在一定pH条件下，可与溴麝香草酚蓝反应生成稳定的络合物这一特性，选择414nm波长处测定其含量。

Methods:total alkaloids of radix Sophora lavescens were extracted by acidified water,then adjusted to neutral by sodium hydroxide solution, dissolved with buffer solution,then were mixed with 0.025％ of bromothymol solution and chloroform,and then the mixture was shaken,standing and demixed,the absorbability of chloroform layer was detected at the wavelength of 413nm.

将苦参的酸水提取液用氢氧化钠溶液调节至中性后，依次加入pH=7.6缓冲溶液，溴代麝香草酚蓝指示液、氯仿混合振摇，静置分层，于413 nm波长处测定氯仿层吸收度。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸－甜菜碱－溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后，再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

Methods:The guinea pig models with choletithasis were established by feeding on speical food induing choletithasis and treated with suspension of JXDC given through gastrotube.After one therapeutic course the total bilirubin,indirect bilirubin,calcium ion (Ca2＋),and β-glucuronidase were detected respectively.

用致石饲料诱发豚鼠胆石模型后，予以金熊胆安胶囊混悬液灌胃治疗，疗程结束后，检测胆汁中总胆红素、间接胆红素、钙离子（Ca2+）含量及β-葡萄糖醛酸酶活性，分别用重氮法、甲基麝香草酚蓝比色法和石井畅法测定。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。