鹅膏属

The results demonstrated that the ITS sequence analysis supported the identification based on morphological characteristics for most of species of Amanita on the species level or on the genus level.

在这种情况下，如能借助分子手段，以传统的形态特征为基本分类依据，辅以DNA序列比较分析，同时借助国际核酸数据库的大量信息资源势必将有助于鹅膏属真菌的准确鉴定。

Abstract:Seven species of Amanita, collected in Lishui of Zhejiang Province, China, were identified primarily based on morphological characteristics. The rDNA ITS regions of these species were cloned and sequenced, and the characteristics of ITS sequences were compared and analysed.

常规对像鹅膏菌属大型真菌的分类鉴定主要是依据子实体的形态，颜色，气味，以及孢子特征等，这些形态特征经常是易变的，模糊的，易混淆的，因而依据传统的形态特征进行分类鉴定总是带有一定的局限性和主观性，要准确鉴定鹅膏属真菌到某一个种实属困难。

The Culture of Green beaus in extract solution of seven poisonous mushrooms resulted in different effects. There are obvious difference among the content of the proteins in Green beaus which were cultured in extract solution of seven poisonous mushrooms, and it is thought that α-amanitin in extract solution of these mushrooms can inhibit the function of RNA polymerase Ⅱ in eukaryoctic cells and further inhibit the synthsis of protein.

用鹅膏菌属含α-毒伞肽的毒素粗提液培养新鲜绿豆，结果在36小时后，各种不同毒苗的毒素粗提液培养的绿豆生长情况有明显不同，用紫外吸收法测定蛋白质含量，发现毒素粗提液培养的绿豆细胞中蛋白质含量比蒸馏水培养的有明显下降，这表明α-毒伞肽的作用机理的确是通过抑制RNA聚合酶Ⅱ而寻致蛋白质合成减少。

ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。