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Key words: Amanita; rDNA; ITS sequence analysis; classification and identification; Macrofungi

本研究在对采自浙江西南部丽水山区的7个膏菌标本进行初步鉴定的基础上,对7个膏菌的rDNA ITS区段进行序列测定和序列特征比较分析,并进一步对ITS序列进行核酸序列数据库GenBank同源性检索比对,构建起系统发育树以期为基于传统形态特征对7个膏菌做出的分类鉴定提供分子依据。

In all, A.subjunquillea.S.lmai methyl alcohol crude extract were research by high pressure liquidchromatography,.separation and purification obtained to 9 kind of compounds, identified 6 kind ofcompounds. The compounds 1~6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin and phalloidin.

总之,黄盖膏甲醇粗提物应用高效液相分离纯化9种单体化合物,鉴定6种分别为β-膏毒肽、α-膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

The translation is over I will seek foreigner to check by myself~Hey Hey~please an English superior help~~ The belly spicy good to eat and delicate beef fresh cow whip of the parsley a round mass of food of the handicraft inside ridge silk of the beef parsley cow meatball of the exquisite article goose bowel exquisite article duck bowel exquisite article hair The coturnix egg cuttlefish Zi the United States fatty cow crab meat fresh goose bowel eel lunch meat green hair belly have no the bone goose Zhang ham the bowel consume the son fish chrysanthemum chicken Zhen The joss-stick bean brain brain flower fresh duck tongue mutton string mutton winding five joss-stick the fatty intestine take fish Huang2 La4 Ding deep sea snow fish crisp meat fresh hair belly waist slice have no bone Feng claw Fresh duck bowel fresh pig Huang2 Hou2 Yu2 the skin Jiao Chang fish 1000 F belly Hong Kong urinate pill fresh squid a fresh fish head shrimp Jiao fresh cow hoof Jin fresh the chicken ridge frailty skin bowel Fresh Huang2 Hou2 deep red shrimp turnip slice big cabbage bean sprout fresh cucumber tree mushroom of the soil bean Feng tail of the bean curd wax gourd Ou slice sea cabbage Tiao powder parsley Soil bean skin fresh duck blood water convolvulus flower of the sea tangle New Year pudding vegetables Tiao skin green bamboo shoot head magnolia slice even mushroom fresh bamboo shoot black mushrooms Gong vegetables frailty bean curd Wild fern vegetables gold needle mushroom chicken leg mushroom milk cow liver germ tea tree mushroom abalone mushroom square Huang2 Ba's eight-treasure rice pudding of the bamboo shoot fried rice with egg Tiao shortcake Lu state Egg crisp Ci Ba pure ll of the butter chafing dish of the oil chafing dish Mi system Mi system pure butter double hotpot of the oil double hotpot Mi system five scented ointment disc The special features vegetables meat dish vegetarian dish a light repast pot bottom oil disc

翻译完了我会自己找外国人核对~嘿嘿~请英文高手帮忙~~精品肠精品鸭肠精品毛肚麻辣牛肉香菜牛肉丸手工里脊丝香菜丸子爽口嫩牛肉鲜牛鞭鹌鹑蛋墨鱼仔美国肥牛蟹肉鲜肠鳝鱼午餐肉绿色毛肚无骨掌火腿肠耗儿鱼菊花鸡胗香豆脑脑花鲜鸭舌羊肉串羊肉卷五香肥肠带鱼黄辣丁深海雪鱼酥肉鲜毛肚腰片无骨凤爪鲜鸭肠鲜猪黄喉鱼皮饺昌鱼千层肚香港撒尿丸鲜鱿鱼鲜鱼头虾饺鲜牛蹄筋鲜鸡脊脆皮肠鲜黄喉大红虾萝卜片大白菜豆芽鲜豆腐冬瓜藕片土豆凤尾海白菜黄瓜木耳苕粉香菜土豆皮鲜鸭血海带年糕空心菜花菜苕皮青笋头玉兰片平菇鲜竹笋香菇贡菜脆豆腐野生蕨菜金针菇鸡腿菇乳牛肝菌茶树菇鲍鱼菇方竹笋蛋炒饭苕酥饼泸州黄粑八宝粥蛋酥糍粑清油火锅秘制牛油火锅秘制全白锅清油鸳鸯锅秘制牛油鸳鸯锅五香碟香油碟特色菜荤菜素菜小吃锅底油碟

Reminded of the Capitoline geese whose cackling alerted the Roman garrison to a night attack by the Gauls in 390 BC, Halifax drily declared:"Rome was saved by geese, but I do not remember that the geese were made consuls."

他想起公元前三九○年卡匹托尔山那些咯咯乱叫,使罗马驻军发觉高卢人乘夜来袭,就淡淡的说:「群救了罗马,但据我所知,那些事后没有封为执政官。

Gosling new type viral enteritis is a new disease, whose epizootiology, the clinical symptoms and lesions are quite similar with those of gosling plague, and causes a serious economy lose for geese industry. Therefore, it is important and necessary to study the etiology, to develope a rapidly diagnosis technique, and to search the prevention and control measure of the disease.

新型病毒性肠炎(gosling new type viral enteritis,GNTVE)是新近出现的一种在流行病学、临床症状和病理变化方面与小瘟(gosling plague,GP)极其相似的传染病,它的出现给养业造成了严重的经济损失。

And then the clever goose finally becomes part of the gaggle and is never lonely again.

所有的都得救了,它们都非常感谢这只聪明的小,于是这只聪明的小从此再也不孤单了,因为他成为了群中的一员啦!

"If a man puts a gosling into a bottle," said Riko,"and feeds him until he is full-grown, how can the man get the goose out without killing it or breaking the bottle?"Nansen gave a great clap with his hands and shouted,"Riko!"

一次,他向一个奇异的禅师南泉请教,是一则关于瓶中之的古老公案,他说:"如果一个人,把一小放在一个瓶子里,而且一直喂养着,直至羽翼丰满,如果不许瓶碎,亦不许杀,那人如何才能让出来?"

This experiment was carried out to study the effect of fiber on growth performance, metabolic rate, digestive tract, meat quality and the change of microbes of intestine in geese of different breeds. The mechanism of utilizing crude fiber for geese was discussed. In this paper, the JL AU I, JLAUIII and JLAU cross geese twenty respective were selected as experimental animals, while Corn stalk and purple medic powder admixture were considered as fiber resource. The test was divided into two periods.

试验选取吉林农大Ⅰ系、Ⅲ系和杂交各20只,以玉米秸和苜蓿混合粉作为日粮纤维源,通过饲养试验、代谢试验、屠宰试验、血清生化指标的测定、组织学观察以及微生物检测等研究了日粮纤维对不同品种生长性能、养分代谢率、消化道、肉质及肠道内微生物变化等的影响,探讨了对日粮纤维利用的机理。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏实验中,雏临床表现为高发病率和高死亡率,食欲和饮水减少,拉白色、黄绿色稀粪,伴有神经症状;常规组织学观察,脾脏和胰腺肿大,有白色坏死斑点,肠道出血和坏死,肝脏肿大变性,肾脏肿大变性有尿酸盐沉着等;用特殊染色和组织化学方法首次对人工感染副粘病毒的雏的重要脏器进行研究,结果表明:胰腺中发现病毒包涵体,肠道中大量纤维素以及胶原纤维增生,肾脏、肺脏大量含铁血黄素沉着,免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少;电镜下多种细胞呈现线粒体水肿,多种实质细胞的染色质固缩,染色质边集,特别是细胞内容物如脂肪滴增多的病理变化特征。

"If a man puts a gosling into a bottle," said Riko,"and feeds him until he is full-grown, how can the man get the goose out without killing it or breaking the bottle?"Nansen gave a great clap with his hands and shouted,"Riko!"

一次,他向一个奇异的禅师南泉请教,是一则关于瓶中之的古老公案,他说:&如果一个人,把一小放在一个瓶子里,而且一直喂养着,直至羽翼丰满,如果不许瓶碎,亦不许杀,那人如何才能让出来?&

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