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A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05~101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明:筛选出的2株杂交瘤细胞,单抗亚类均为IgG1型,其中4G1-B3株的ENR mAb间接ELISA效价为1:1.024×106,亲和常数为9×1010L/mol;竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L,与其他化合物的交叉反应率均小于0.01%,肉样、肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%,不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小,试剂盒保存期6个月以上。

Almost extinct large mammals; mammals are leopard, wolf, fox, badger, dog, hare, weasel, hedgehog, mink, etc.; birds are sparrows, pigeons, magpies, capital verification, owls, eagles, pheasants, stone chickens, crows, quail, woodpecker, cuckoo, thrush, oriole, etc.; fish carp, grass carp, crucian carp, silver carp, loach, herring, etc.; reptiles are turtles, soft-shelled turtle, gecko, etc..

较大的兽类几近绝迹;哺乳类有豹、狼、狐、獾、狸、野兔、黄鼠狼、刺猬、水貂等;鸟类有麻雀、鸽、喜鹊、验资、猫头鹰、雕、雉、石、乌鸦、鹌鹑、啄木鸟、布谷鸟、画眉、黄鹂等;鱼类有鲤鱼、草鱼、鲫鱼、鲢鱼、泥鳅、青鱼等;爬行类有龟、鳖、壁虎等。

Chicken, duck, goose, beef, pork, rabbit, sika deer, turtle dove, owl, Chinese Bamboo Partridge, magpie, common pheasant, crane, and various types of fish were commonly consumed meats.

,鸭,鹅,牛,猪,兔,梅花鹿,斑鸠,猫头鹰,竹,喜鹊,雉,鹤以及各种鱼类都是常见的食用肉类。

As the Japanese, the "bird" the Chinese alone usually refers specifically to this bird means chicken, it translated into Chinese Torii is actually a "chicken aircraft" means.

由于在日语中,&鸟&这个汉字单用时,通常是专指这种鸟类之意,因此译为中文鸟居其实是&架&的意思。

So get rid of the lone chicken wing and neck; only feed those if, and ONLY IF, they are attached to half of a breast, half of a chicken, or a whole bird.

所以不要单独喂食长长的脖子或翅,但与半个或这整只鸟相连的时候可以食用

Few studies about the flavor of chicken broth were reported.

目前国内外对肉汤特别是老母汤香味的研究很少,研究肉汤香味的组成和含量以及风味物质的形成机理有重要意义。

In the most recent case she served some chicken broth , which was as thin as plain drinking water.

最近一次,上来的汤淡得像白开水,我跟汪先生说:'这不是煮过的汤,只像在里面洗过一次澡。

In the most recent case she served some chicken broth, which was as thin as plain drinking water.I

最近一次,上来的汤淡得像白开水,我跟汪先生说:'这不是煮过的汤,只像在里面洗过一次澡。

The chickens made an airplane to leave the chicken house finally, was only left over the dumbfounded chicken farmer.

们终于做了一架飞机离开了场,只剩下目瞪口呆的农。这个故事告诉我们,只要肯努力,不灰心,事情总会成功的

Grains. Wild grass is an ideal base for chicken farming, chickens foraging between the desirability of a large number of really nutritious green leaves.

杂粮。野生小草,是理想的土养殖基地,只觅食真间可取大量营养丰富的绿叶。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。