鳞茎叶

Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

Using bulblet and scale segments of Asiatic Lilium 'Mona' in vitro, the effects of MeJA on the growth of scale leaves were investigated.

方法］以亚洲型"Mona"百合试管鳞茎、鳞片的切片为外植体，研究茉莉酸甲酯对鳞片叶及鳞茎生长的影响。

Mean bulblet diameter was 5-10 mm made the ratio was 97~100%.Bulb with leaves and roots can make the ratio higher.

试管鳞茎的直径为5～10mm时，移栽成活率高达97％～100％，并且鳞茎带叶和根有助于移栽成活。

The differentiation ability for different parts of bulblet leaves was studied,and the results showed that the apical part of leaves did not regenerate,however,mass shoot regeneration occurred from the leaf base,occasionally from the middle part.

通过对再生苗叶片不同部位的再分化实验，比较得出小鳞茎叶的叶片基部分化能力非常强，而叶片上部基本不分化，鳞茎叶基部是建立高频快速再生体系的首选材料。

The research aimed to study the effects of methy jasmonate on bulblet and scale leaf growth of Lilium 'Mona' in vitro, to solve vigorous growth of scale leaves during tissue culture of bulblets in Asiatic Lilium.

［目的］研究茉莉酸甲酯对组培"Mona"百合鳞茎及鳞片叶生长的影响，以解决亚洲系统百合在试管鳞茎培养过程中鳞片生长过旺的问题。

Application of MeJA is the efficient way for overcoming the problem of subculturing overelaborate during the tissue culture of Lilium 'Mona', and 1.0 μmol/L MeJA is suitable for promoting bulblet growth and inhibiting scale leaf growth of Lilium 'Mona'.

结论］在"Mona"百合试管鳞茎培养的培养基中加入1.0μmol/L的茉莉酸甲酯时即可抑制鳞片叶生长也可促进鳞茎膨大生长。

Result The scale leaf formation was inhibited by MeJA, the higher the concentration of MeJA the more efficient for restraining scale leaf growth. 100.0% of bulblet formation was obtained in the treatment of 1.0 μmol/L MeJA, but the scale leaf growth was significantly lower than control. 3.0 μmol/L MeJA had the inhibitory effect to both the bulblets and the scale leaves.

结果］茉莉酸甲酯对"Mona"试管百合鳞片叶生长有抑制作用，浓度越高其效果越明显；当茉莉酸甲酯浓度为1.0μmol/L时，鳞片叶发生率(87.9%)明显低于对照(100.0%)，但鳞茎分化和生长也显著好于对照；但茉莉酸甲酯浓度超过3.0μmol/L时，不仅对鳞片叶而且对鳞茎生长也有抑制效果。

In order to breed new germplasm for Narcissus tazetta,the bulb of Narcissus tazetta is used as explants.The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied.Three materials,including the bulb basal plate tissues inoculated for 7 days,tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days,and small bulbs in vitro,are studied.

以带鳞片叶的鳞茎盘为外植体，接种在添加激素的MS培养基上，通过激素组合筛选合适的培养基获得不定芽，同时对在预分化培养基上接种7D的外植体、接种25D后转接到分化培养基上3D的外植体、诱导产生的试管小鳞茎进行辐照诱变处理，研究了60COΓ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

In cider to breed new gern1asm for Narcissus tazetta, the bulb of Narcissus tazetta is used as explants. The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied. Three materials, including the bulb basal plate tissues inoculated for 7 days, tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days, and small bulbs in tarn, are studied.

以带鳞片叶的鳞茎盘为外植体，接种在添加激素的MS培养基上，通过激素组合筛选合适的培养基获得不定芽，同时对在预分化培养基上接种7d的外植体、接种25d后转接到分化培养基上3d的外植体、诱导产生的试管小鳞茎进行辐照诱变处理，研究了60Coγ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

Using the scanning electron microscope, the starch granules were observed which gathering and stuffing in scale cell during bulb development.

用扫描电镜观察到鳞片细胞中明显的淀粉颗粒，并随鳞茎的发育充满整个细胞腔。14C示踪技术分析表明，含苞期的14C-同化物分配以地上部为主，集中在花苞部位；花后期的14C-同化物分配以地下鳞茎为主，其中下位叶标记处理的鳞茎分配量占植株总14C的比例达85.8%，证实了植株的光合产物在花后期主要供应鳞茎发育膨大所需。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。