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The bulbs of the Lycoris radiatat were pretreated with comminution, ultrasonic and basification methods. The effect of the pretreatment was studied, via observing microscopic character of the cell wall fragmentation of Lycoris radiatat and the yield of galanthamine with SC-CO2 extraction.

采用粉碎、超声波和碱化预处理方法处理石蒜鳞茎原料,通过对原料细胞壁的破碎状况的显微特征观察,以及SC-CO2提取加兰他敏提取率的变化考察了预处理的效果。

Low temperature treatment;(3) Different parts of a bud scale, the lower parts, the higher differentiation frequencies;(4) Growth regulator, the most adapta.

百合的9 个优良品种快速繁殖条件优化的研究表明,不同百合品种的鳞片分化小鳞茎能力有一定差异,高者达 86。

Five concentrations of colchicines were used to treat aerial bulbil in garlic inflorescence for polyploid induction in vitro.

用5种不同浓度的秋水仙素处理大蒜花苞气生鳞茎进行多倍体诱导,发现在固体培养基上接种处理时毒害作用较小,四倍体的诱导率最高能达到66。

It is found that the hazard of colchicines to aerial bulbil is much less in solid media than in liquid media,and the highest induction rate of tetraploid garlic is 66.7% in solid medium.

用5种不同浓度的秋水仙素处理大蒜花苞气生鳞茎进行多倍体诱导,发现在固体培养基上接种处理时毒害作用较小,四倍体的诱导率最高能达到66.7%。

The results showed that NAA at 0.15~0.9 mg·L -1 was appreciate, and 0.69mg· L -1 was the optimum concentration for bulbil induction from scale expl ants in vitro; and that 3.2mg.L -1 was the optimum concetration of 2,4ˉD for callus induction from l eaflet explant in vitro.

结果表明, NAA诱导离体鳞片外植体产生小鳞茎的用量范围为 0.15~ 0.9 mg·L -1 ,最佳用量为0.69 mg·L -1 ; 2,4ˉD诱导叶片形成愈伤组织的最佳用量为3.2 mg· L -1 。

Adopting orthogonal polynomial regression method,the mathema tical model of the effects of NAA on bulbil induction, and that of 2,4ˉD on callus induction f rom leaflet on oriental lily cv."Tiber" were established.

借助正交回归多项式建立数学模型的方法,研究了NAA对东方百合"蒂伯"鳞片诱导小鳞茎及2,4ˉD对小叶片诱导愈伤组织的影响,以探讨东方百合组织培养中NAA及 2,4ˉD 的用量范围及最佳用量。

It took 180 days from planting to bulbil differentiation, in which stage the accumulation of dry matter and uptake of N, P and K everyday were less. It took 52 days from bulbil differentiation to harvest, in which stage scape growed and bulb extended, the accumulation of dry matter and uptake of N, P and K everyday increased rapidly.

从播种到鳞芽花芽分化期(180d),大蒜干物质日均积累量相对较低,对氮、磷、钾的吸收量分别占整个生育期的30.7%、36.8%和46.1%;从鲜芽花芽分化期到收获(52d),蒜薹伸长、鳞茎膨大,大蒜干物质日均积累量迅速增加,对氮、磷、钾的吸收分别占整个生育期的69.3%、63.2%和53.9%,到收获前期干物质积累和养分吸收达到高峰。

The research aimed to confirm the suitable hormone kinds and level for rooting induction of bulblet in tissue culture seedlings of narcissus.

[目的]确定水仙组培苗小鳞茎生根诱导的适宜激素种类和水平。

The test-tube plantlet was formed after rooting, through the virus detection on test-tube plantlet, the plant without virus was selected out and the bulblet was obtained through transplanting.

生根后形成试管苗,通过时试管苗的病毒检测,选择不带病毒的植株,移栽得到小鳞茎

Using the tissue cultured bulblet of narcissus cultivar Fortissimo as tested materials, with 1/2MS as the basic medium, 4 conch, of NAA and IBA including 1.0, 0.5, 0.1 and 0.05 mg/L were set up to study the effects of hormone on rooting induction of tissue culture seedlings in narcissus.

方法]以水仙栽培品种Fortissimo的组织培养小鳞茎为试验材料,以1/2MS为基本培养基,设计1.0、0.5、0.1,0.05mg/L 4个NAA、IBA浓度,研究激素对水仙组培苗生根诱导的影响。

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