鲽

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA，并采用RT-PCR方法，根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物，克隆了含开放阅读框的生长激素(Growth Hormone，GH)cDNA序列，测序结果显示，高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp，编码140-170个氨基酸的成熟GH多肽片段；从漠斑牙鲆和大菱鲆脑垂体中提取mRNA，利用SMART-RACE技术建立脑垂体cDNA文库，并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。

Phylogenetic and molecular evolutionary analyses of 13 mitochondrial coding genes conducted using MEGA version 3.0 showed that all the pleuronectiform species except for soleid species which had close phylogenetic relationship with perciformes and the soleid species had far evolutionary relationship with all other fish species. These new data provide a useful tool for many research areas, i. e. evolutionary study and identification of spotted halibut.

用NJ法和MP法对5个目22种鱼mtDNA的13个蛋白质编码基因的氨基酸序列进行系统分析，结果显示，圆斑星蝶与石鲽关系最近，鲽形目的鲆、鲽类与鲈形目的够科、鲷科鱼类亲缘关系较近，而同属于鲽形目的塞内加尔鳎没有和任何目聚在一起，成为一个独立的分支。

Phylogenetic and molecular evolutionary analyses of 13 mitochondrial coding genes conducted using MEGA version 3.0 showed that all the pleuronectiform species except for soleid species which had close phylogenetic relationship with perciformes and the soleid species had far evolutionary relationship with all other fish species. These new data provide a useful tool for many research areas, i. e. evolutionary study and identification of spotted halibut.

用NJ法和MP法对5个目22种鱼mtDNA的13个蛋白质编码基因的氨基酸序列进行系统分析，结果显示，圆斑星鲽与石鲽关系最近，鲽形目的鲽、鲽类与鲈形目的鲹科、鲷科鱼类亲缘关系较近，而同属于鲽形目的塞内加尔鳎没有和任何目聚在一起，成为一个独立的分支。

The result was consistent with morphology of Pleuronectiformes on the whole.In the topology founded,Scophthalmus maximus and Solea senegalensis formed an independent branch each other and showed far relationship with species of Bothidae and Pleuronectidae,the phylogenic position of Solea senegalensis is accorded with the reported result of analysis made by mitochondrial genome.

运用PAUP软件对15种鲽形目鱼类与另外7种不同种属鱼类进行了分子系统进化树分析，结果与根据传统的形态学和生化特征分类进化地位基本一致，在15种鲽形目鱼类中只有大菱鲆和塞内加尔鳎各自单独形成一个分支且与鲆科和鲽科鱼类相距较远，其中塞内加尔鳎等种类的分类地位与根据线粒体DNA序列分析的系统发生模式基本吻合。

Any of various flatfishes,chiefly of the genera Limanda and Hippoglossoides, related to and resembling the flounders.

比目鱼，孙鲽一种比目鱼，主要是黄盖鲽属和拟庸鲽属的鱼类，与鲽鱼有关而且很相似

"Dab:any of various flatfishes, chiefly of the genera Limanda and Hippoglossoides, related to and resembling the flounders."

比目鱼，孙鲽：一种比目鱼，主要是黄盖鲽属和拟庸鲽属的鱼类，与鲽鱼有关而且很相似。〞

Any of various flatfishes,such as Hippoglossoides platessoides of North american Atlantic waters,related to the plaice.

欧鲽其它生长在北美洲大西洋水域中比目鱼，如美洲拟庸鲽，与欧鲽有亲缘关系

Any of various flatfishes,chiefly of the genera Lim and a and Hippoglossoides,related to and resembling the flounders.

比目鱼，孙鲽一种比目鱼，主要是黄盖鲽属和拟庸鲽属的鱼类，与鲽鱼有关而且很相似

Based on the crossing data among these flatfishes, the relationship between pairwise genetic distance and hybrid fitness was evaluated. The findings indicated that positive relationship might exist between genetic distance and hybrid fitness. The pairwise genetic divergence between species decreased as the interspecific hybrid fitness largened. These results implied that speciation clock might exsit in Pleuronectiformes.

根据这几种鲆鲽鱼的杂交是否可行的试验结果，评价种间遗传距离与杂交可适度的关系，结果表明，这8种鲆鲽鱼类的种间遗传距离与杂交可适度呈显著的负相关(r2 = 0.805，P 0.01)，即种间遗传分化越大，杂交成功的可能性越小，这表明鲆鲽鱼类中可能存在物种进化的不亲和钟。

Any of numerous chiefly marine fishes of the order Pleuronectiformes, including the flounders, soles, and halibuts, having a laterally compressed body with both eyes on the upper side .

比目鱼鲽形目的各种主要海水鱼中的一种，包括鲽鱼、鳎鱼和庸鲽，长有侧扁的身体，两眼位于上侧

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。