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鲎试剂

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To study the changes of plasma endotoxin NO and hemorheology in experimental cirrhotic rats and their correlation,we have used hemorheology analysis system and the means of limulus amoebocyte lystata assay Griess assay to detect changes of plasma endotoxin NO and hemorheological parameter in rats at 3 6 9 weeks after CCl4 administration.

为探讨肝硬化中血浆内毒素、NO水平的变化及血液流变学特性的改变及其相互关系,我们通过用四氯化碳(carbon tetrachloride,CCl_4)来诱导大鼠肝硬化模型,采用鲎试剂动态浊度法、镉还原比色法、血液流变学分析仪,分别检测了大鼠肝硬化演变过程中早期(诱导第3周末)、中期(诱导第6周末)、晚期(诱导第9周末)的血浆内毒素、NO水平及血液流变学各参数。

The sensitivity of each new batch of limulus used in testing shall be calibrated before the test.

每批新鲎试剂在用于试验前都要进行灵敏度的标定。

OBJECTIVE:To compare the results of the bacterial endotoxins test between two Limulus Amebocyte Lysate reagents.

目的 :比较两种鲎试剂的细菌内毒素检查结果,避免判断误差。

Results: The ofloxacin injection after4 times dilution had no interference to limulus reagent reaction.

结果:氧氟沙星注射液4倍稀释后对鲎试剂反应无干扰。

1 The sensitivity of limulus batch to be used shall be determined and confirmed to comply with the requirements before the test.

2.5.4.2.1试验前应该对使用批号鲎试剂的灵敏度,应符合规定。

By using the limulus test the plasma endotoxin of 20 cases of malignant obstructive jaundice was determined before and after different bile duct drainage.

利用鲎试剂与鲎三肽的偶氮显色法测定20例恶性梗阻性黄疸患者不同引流手术前后血浆内毒素水平。

Objective To establish a feasible method for purifying tachyplesin from the wastes of tachypleus amebocyte lysate.

目的 建立从鲎试剂生产废料中纯化鲎素的方法。

Methods:By counterchecking the ∧ of Limulus Amebcyte Lysate and determining the L of the showpiece of the bacterial endotoxins ,interference test of the showpiece,we decide to use the limulus test to check the bacterial endotoxins of the showpieces.

通过对鲎试剂标示灵敏度的复核,以及样品细菌内毒素限值的确定,样品的干扰试验,从而运用鲎试剂对样品中的细菌内毒素进行检测。

Take several burettes of limulus reagent of the same batch and respectively dissolve them into limulus solution as per labeled amount by adding the water free of pyrogen.

取同一批号鲎试剂若干支,分别按标示量加入无热原水溶解为鲎试剂溶解液。

The results indicated that the Nao Weizhi Injection diluted properly had no interference in TALT.So the TAL could be used to test the bacterial endotox in the Nao Weizhi Injection and substitute RT for the pyrogens test.

结果表明,脑维治注射液经适当稀释对用鲎试剂检查其中的细菌内毒素无干扰,鲎试剂法可以用于脑维治注射液细菌内毒素检测,以替代家兔法检查热原。

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