鲎

the determination of bacterial endotoxin can be used to replace the detection of pyrogen in sodium cefoperazone injection.

选择适宜灵敏度的鲎试剂，注射用头孢哌酮钠的热原检查可用细菌内毒素检查法代替家兔法。

Methods Bacterial endotoxin was inspected using the method in specified the Addenda of China Pharmacopoeia( second part ,2005 edition).

方法以不同厂家的鲎试剂对3批华蟾素注射液分别进行干扰试验。

The method of the soluble peptidoglycan isolation by short dose benzylpenicillin sodium and sephadex G-100 was founded; through the SLP reagent kit, the limulus test and the improved LOWRY reagent kit detecting, the prepared soluble peptidoglycan include shorter LPS and lower protein, its purity coefficient reach 93%.

建立并完善了青霉素诱导和Sephadex G-100凝胶柱法制备金黄色葡萄球菌可溶性肽聚糖的方法；经SLP试剂、鲎试验及改良的LOWRY试剂检测，所制备的金黄色葡萄球菌可溶性肽聚糖中LPS和蛋白的污染均较少，纯度达到93%以上； 3。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

Used in some classifications; includes the orders Xiphosura and Eurypterida.

用在某些分类中；包括剑尾目和广翅鲎目。

She spent several years doing research in the Department of Polymolecular Biology of the Kyushu University under the guidance of Prof.S.

她在国际知名的鲎血液研究专家岩永贞教授的指导下，在九州大学的高分子生物部门度过了几年的研究生涯。

Type genus of the family Triopidae: small crustaceans with a small third median eye .

鲎虫科的模式属；头及胸部覆一卵形钝状壳及有一第三中眼。

Type genus of the family Triopidae: small crustaceans with a small third ''.'median'.''''.

鲎虫科的模式属；头及胸部覆一卵形钝状壳及有一第三中''。

Objective :To quantify bacterial endotoxin in the raw materials of tobramycin for injection by dynamic turbidimetric Limuius test.

目的：应用动态浊度法鲎试验定量测定妥布霉素原料药中细菌内毒素。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！