鱼藤素

Toxicity of four compounds identified as 6a, 12a-dehydro-α-toxicarol, 6a, 12a-dehydro-deguelin,-tephrosin and-6-hydroxy-6a, 12a-dehydro-toxicarol obtained from the leaves and stem of Amorpha Fruticosa and their mixture in a natural ratio 1:2.54:4.18:2.54 were tested against Trematodes grandis.

通过对紫穗槐叶和茎成分分离，得到6a，12a-脱氢－α-灰叶酚、6a，12a-脱氢鱼藤素、灰叶素、6-羟基－6a，12a-脱氢灰叶酚4个化合物。

Toxicity and repellent activity of several plant-based compounds on Blattella germanica were studied, results showed that rotenone, toosendanin and azadirachtin had stomach toxic effect on Blattella germanica, rotenone showed high stomach toxicity and all insects died after 8 hours, matrine also has deformative effect on Blattella germanica , matrine and toosendanin can affect oviposition of Blattella germanica; repellent activity of these botanicals was further studied, results showed that bering hydrochloridum, azadirachtin and sparteinehave had repellent activity against Blattella germanica, observation also showed that rotenone and bering hydrochloridum had high contact toxicity against Blattella germanica, the time of all insects knocked down were 8 and 48 hours.

选取了7种植物源化合物，测定了它们对德国小蠊的毒性和驱避性，结果表明，鱼藤酮、川楝素和印楝素对德国小蠊具有不同程度的胃毒作用，其中鱼藤酮乙酸乙酯溶液作用较强，8小时死亡率已达100％，川楝素和印楝素对德国小蠊也表现出一定的毒杀作用，观察还发现印楝素和苦参碱对德国小蠊的发育有致畸作用，苦参碱和川楝素影响其卵鞘形成；进一步对这几种化合物的驱避性进行研究，结果表明盐酸黄连素、鱼藤酮乳油和印楝素乳油对德国小蠊有不同程度的驱避作用，其驱避率分别为96.7％、51.3％和50.1％，另外观察到鱼藤酮乙酸乙酯溶液和盐酸黄连素对德国小蠊具有较强的触杀活性，供试虫全部被击倒的时间分别为8小时和48小时。

The study was purposed to investigate the effect of deguelin on expression of nucleoporin 98(nup98) in leukemia K562 cells.

本研究观察鱼藤素对白血病细胞株K562细胞核孔蛋白nup98表达的影响。

Results:(1) The proliferation of Jurkat cells was inhibited by deguelin in a time- and dose-dependent manner, with IC50 value for 24 h being 46.03±0.13 nmol/L.

2鱼藤素以剂量依赖性方式诱导Jurkat细胞凋亡，40 nmol/L的鱼藤素处理24 h后出现明显的DNA剪切现象，伴有典型的凋亡细胞形态学改变，其总凋亡率达(19.87±1.76)%。

Effects of deguelin on the regulation of nucleoporins and nucleophosmin in Jurkat leukemia cells in vitroObjective: To study the antileukemia effects of deguelin on leukemia cells, and explore the possible mechanisms to provide more theories for clinical application.

第一部分鱼藤素对Jurkat白血病细胞核孔蛋白与核磷蛋白的调节作用目的：研究鱼藤素对白血病细胞增殖和凋亡的影响，并探讨其可能的作用机理，为其临床应用提供理论基础。

Deguelin induced DNA fragmentation and apoptotic cell death in a dose-dependent manner, which was accompanied by typical morphological changes associated with apoptosis.

3鱼藤素能同时在蛋白和基因水平，以剂量依赖性方式抑制核孔蛋白Nup88、Nup214以及核磷蛋白NPM的表达。

Objective To investigate antitumor activity and molecular mechanism of deguelin to the human U937 leukaemia cells and to explore the mechanisms regulating cell cycle and nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro.

目的研究鱼藤素对人类白血病细胞株U937细胞体外抗肿瘤作用，研究其引起细胞周期与核孔蛋白Nup98和Nup88的改变，探讨其抗癌作用的分子机制。

MTT assay was used to assess the effects of deguelin on cell proliferation. FCM and RTPCR were used to analyze the changes of nup98 mRNA and protein in K562 cells after treating with deguelin.

用MTT比色法检测细胞增殖活性；流式细胞术及RTPCR法分析鱼藤素作用K562细胞后nup98蛋白及mRNA含量的变化。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失