鱼藤

MTT assay was used to assess the effects of deguelin on cell proliferation. FCM and RTPCR were used to analyze the changes of nup98 mRNA and protein in K562 cells after treating with deguelin.

用MTT比色法检测细胞增殖活性；流式细胞术及RTPCR法分析鱼藤素作用K562细胞后nup98蛋白及mRNA含量的变化。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

However, the role of microglia in rotenone-induced neurodegeneration has not been reported.

但是，在鱼藤酮诱导的多巴胺神经元变性过程中，小胶质细胞的作用尚未可知。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

The content of rotenone in leaves of Tephrosia vogelii and the extract time were determined by extracting purple variety leaves of T.

用苯和氯仿作溶剂，采用冷浸法，对紫花非洲山毛豆叶片进行多次提取，测定了非洲山毛豆叶片中鱼藤酮的含量和提取时间。

Seasonal Variations and Analysis of Rotenone Content in Tephrosia vogelii Hook f .;2. Three kinds of extracting methods and six kinds of solvents were applied to extract the leaves of Tephrosia vogelii Hook f .

用索氏、冷浸和振荡抽提 3种方法和 6种不同的溶剂对非洲山毛豆叶片进行了提取研究，并用HPLC测定了提取物和植物叶片干粉中鱼藤酮的含量。

Topically treated with acetone extracts of the leaves of Tephrosia vogelii Hook f .

用非洲山毛豆叶丙酮提取物或鱼藤酮点滴处理菜粉蝶幼虫，出现畸形幼虫和畸形蛹。

Tephrosia vogelii is all treasures, not only can extract the root, stem or leaf to rotenone, but not strict on the environment, can make use of sloping land, wasteland cultivation, the roots contain rhizobia, can be free of fixed nitrogen in the air, can play to improve The role of the soil.

非洲山毛豆浑身是宝，不仅其根茎叶皆可提取到鱼藤酮，而且对环境要求不严，可利用坡地、荒地种植，其根部含有根瘤菌，可以固定空气中的游离氮，可起到改良土壤的作用。

The Bioassay of rotenone and Tephrosia vogelii extracts aerosols to Culex pipiens quinquefasciatus, Blattellagermanica were researched with electron sprayer.

用电子喷雾装置测定了鱼藤酮和非洲山毛豆提取物气雾剂对致倦库蚊和德国小蠊的击倒活性。

RESULTS Specific inhibitors of respiratory chain complexes Ⅰ,Ⅱ,Ⅲ, and Ⅳ of rotenone, thenoyltrifluoroacetone, antimycin A and potassium cyanide, and the mitochondrial uncoupler carbonyl cyanide mchlorophenylhydrazone resulted in obvious changes of intracellular ROS production.

结果 呼吸链复合物Ⅰ，Ⅱ，Ⅲ，和Ⅳ抑制剂鱼藤酮、噻吩甲酰三氟丙酮、抗霉素A、氰化钾及氧化磷酸化解偶联剂羰氰苯腙能够明显改变But所致细胞内活性氧的产生。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。