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高香草醛

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5 % vanillin acetum and perchloric acid as color developer, the content of ursolic acid of kudingcha growed in Hainan province was determined at 548 nm.

用5%香草醛冰醋酸及高氯酸显色,在波长548nm处用分光光度法测定了海南苦丁茶中的熊果酸含量。

objectiveto determine the content of the tritepenoidic acid in dracocephalum moldavica l .methodswith oleanolic acid as the standard,5% vanillin-acetic acid and perchloric as the developer,colorimetric method was applied.

目的测定香青兰全草中总三萜酸成分含量。方法以齐墩果酸为指标,采用香草醛-冰醋酸及高氯酸显色后用分光光度法测其含量。

MethodsVanillin was used as colour developing reagent in the presence of perchloric acid.

方法利用在高氯酸的条件下香草醛-冰醋酸与三萜酸的反应测定总三萜酸。

objective:to make a comparative analysis of the total saponins content in polygalaceae phloem and xylem parts to guide clinical treatment.methods:we determined the polygalaceae total saponins content at the 575 nm with oleanolic acid as a reference substance and vanillin-perchoric acid as a developer by using uv-visible spectrophotometry.results:the total saponins content of xylem parts was 3.33%,and that of polygalaceae phloem was 0.69%.conclusion:the total saponins content in polygalaceae xylem parts is much more than that of phloem parts,the total saponins in polygalaceae mainly exists in xylem parts.

目的:对远志原药材木部及皮部中总皂苷含量进行比较分析,用以指导临床用药。方法:以齐墩果酸为对照品,香草醛-高氯酸为显色剂,采用紫外-可见分光光度法在575 nm处测定远志总皂苷含量。结果:以此方法对远志木部及皮部中总皂苷含量进行比较、分析,为远志皮部总皂苷含量为3.33 %、木部总皂苷含量为0.69 %。结论:远志中皮部总皂苷含量远远大于木部,远志总皂苷主要存在于根皮中。

The results showed that in the growth period of R. glutinosa, soil ferulic acid, vanillic acid, and vanillin contents had a persistent increase, while β-hydroxybenzoic acid content increased first but decreased then. The addition of ferulic acid (8 μg·ml-1), vanillic acid (08 μg·ml-1), vanillin (12 μg·ml-1), and β-hydroxybenzoic acid (30 μg·ml-1) in water culture had stronger inhibitory effects on R. glutinosa root length, root mass, fresh plant mass, and plant height. Treating with test phenolic acids except β-hydroxybenzoic acid increased leaf chlorophyll content significantly, compared with the control. After treated with test phenolic acids, plant SOD and POD activities decreased after an initial increase, and plant malondialdehyde content increased. In the treatment of ferulic acid, root SOD and POD activities were the lowest, and roots were rotted and dead.

结果表明:阿魏酸、香草酸和香草醛含量在地黄生长期表现为持续增加,而对羟基苯甲酸含量先升高后降低;外加阿魏酸(8 μg·ml-1)、香草酸(0.8 μg·ml-1)、香草醛(1.2 μg·ml-1)和对羟基苯甲酸(3.0 μg·ml-1)对水培地黄根长、根质量、全株鲜质量和株高有较强的抑制作用;酚酸处理使地黄叶片叶绿素含量降低,除对羟基苯甲酸外差异性极显著;经过酚酸处理的地黄幼苗SOD和POD活性为先升高后下降,丙二醛含量升高,其中阿魏酸的处理使根中酶活性降至最低,根部腐烂死亡。

The vanillin-perchioric acid was used as the chromogenic agent, and glacial acetic acid was used as solvent system.

选取香草醛-高氯酸作为显色剂,冰醋酸为溶剂。

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