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Insititute of Refrigeration and Cryogenics, ShanghaiJiaoTongUniversity, Shanghai, 20030, China

采用计算机仿真方法[1-2]为换热器的高效设计提供了必要的手段。

Having Been studied and analyzed by cryptologist for over 10 years all over the world, PGP algorithm has been regarded as of security, high efficiency and operability. Consequently, series of PGPs standard for application to protect E-mail came out.

经过十多年世界范围的应用研究和密码分析家们的分析,PGP被认为是安全、高效和易于实现的加密算法,形成了应用于保护电子邮件的一系列标准,并且有相当多的商业软件和免费软件被相继开发。

The eels were exposed to crystal violet bath water of 0.1 mg/L for 24 h at a water temperature varying between 18~21℃.

在水温18~21℃,结晶紫浓度为0.1mg/L的条件下,对鳗鲡进行24h药浴后,采用高效液相色谱-串联质谱仪,对鳗鲡肌肉组织中的结晶紫和隐色结晶紫含量进行连续56d的监测。

Therefore, the development of a new crystallization technology with independent intellectual property rights and the design of a continuous column crystallizer with high efficiency, have a very important significance.

因此,开发具有自主知识产权的新型结晶分离技术,设计一个高效连续的熔融结晶装置就具有非常重要的意义。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a,构建重组质粒 pET-20b-bglA 和 pET-28a-bglA,然后转化不同大肠杆菌 E.coli 宿主,Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达,重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA),可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA),比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA),这些结果表明,E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因,分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别,有助于提高该酶在 E.coli 中的表达;进一步优化诱导条件,重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养,IPTG 浓度由 1000 μM 降至 25 μM,目的蛋白可溶性表达由 12.3%增至 32.8%,提高 1.7 倍,16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达,较 37 ℃下诱导培养提高 1.2 倍。

The efficient visibility culling method is one of the important research aspects of real time rendering of complexle dynamic scene.

对复杂动态场景进行高效的可见性裁剪是实时绘制领域研究中的一个重要问题。

With the development of molecular biology, people find another way of cultivar new breeds.

植物耐旱分子机制的研究和生物技术的日臻完善,为培育高效耐旱植物迎来了曙光。

In the conclusion, fair and square of the judicatory is the key connotation of the restructure of the global judicial system. And it is the main content of the field of judgement from the intendance of WTO. So only to build up an open and efficient judicatory on the basis of tradition culture trait is China's rational choice.

总之,入世条件下的中国司法公正、司法独立是我国进行司法现代化建设的重要价值目标,也是WTO组织对于成员方域内法院审判工作的主要监督内容,只有建立以中国传统司法文化为基础的高效、开放的司法制度文化,才是我国在全球化司法重构中的理性选择。

In the conclusion, fair and square of the judicatory is the key connotation of the restructure of the global judicial system. And it is the main content of the field of judgement from the intendance of WTO. So only to build up an open and efficient judicatory on the basis of tradition culture trait is the Chinas rational choice.

总之,入世条件下的中国司法公正、司法独立是我国进行司法现代化建设的重要价值目标,也是WTO组织对于成员方域内法院审判工作的主要监督内容,只有建立以中国传统司法文化为基础的高效、开放的司法制度文化,才是我国在全球化司法重构中的理性选择。

The analysis of oxidation products of cumene and m-diisopropylbenzene was performed on the high performance liquid chromato graphy.

首先建立了高效液相色谱分析异丙苯与间二异丙苯氧化产物的方法。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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