高效

Efficient imple- mentation, must be a professional operations team, and scientific and efficient implementation of the model anchored.

高效执行，必须以专业运作团队与科学高效的执行模式为基点。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本实验通过RT-PCR从番茄花粉中获得高效特异表达的高赖氨酸基因TSB的cDNA序列，构建其受控于单子叶植物Ubil强启动子下的高效表达质粒pBIUB-TSB。

Each of the two genes was expressed by high-efficient expression-vector pBV220, two expression products were of Chorismate mutase/Prephenate dehydratase or DAHP synthetase activities.

用高效原核表达载体pBV220分别实现了分支酸变位酶／预苯酸脱水酶和抗反馈抑制的DAHP合成酶的高效表达，两种表达产物都具有生物学活性。

In this paper, the structural types, performance characteristics and the situations of research and application of commonly used superplasticizers at home and abroad were introduced in detail. The development condition and the problems in practice of Naphthalene series supeiplasticizer were also analysed.

本文详细介绍了常用高效减水剂的结构类型、性能特点及在国内外的研究应用现状；分析了萘系高效减水剂的发展状况及应用中存在的问题。

Using highly efficient water saving toilets the water consumption can be minimized at source and the highly concentrated night soil can be separately collected.

通过高效的节水便器，一方面从冲厕用水上节水，另一方面单独收集高浓度的人粪尿，在达到高效节水的同时将人粪尿从废水中分离。

A series of ultrajets are developed, such as 1MD3 perforating gun, inner blind hole perforating gun, unconfined perforating gun, and so on. A series of efficient perforating technology are also optimized and assembled, such as recombination perforating, orientable perforating, tubing conveyed perforating and production string combination operation technology, and so on.

结合大庆油田薄差层的地质特征，开发出了1MD3射孔器、内盲孔射孔器、无围压射孔器等高效射孔器，同时优选配套了包括一体复合射孔工艺技术、定方位射孔工艺技术、选发射孔工艺技术等系列高效射孔工艺技术。

Separation and purification of microorganism, advanced wastewater treatment technology, RO technology (especially for various anti-pollution and low-press reverse osmose membrane), as well as other new physical and chemical sewage treatment technology, phosphorus and nitrogen desorbed from living waste technology, new type of electrochemistry sewage treatment technology and special electrode development.

污水生化处理高效微生物分离提纯，污水深度处理技术，膜过滤技术（特别是各种高通量抗污染低压反渗透膜），以及其它新的污染物高去除率污水物理化学处理技术，生活污水磷、氮高效脱除技术，新型电化学污水处理技术与特殊电极研发。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The effect of a variety of experimental parameter has been examined with respect to the performance of membrane bed for removing water from fuel .

对研制的聚结分离床进行了油品脱水的实验研究，结果表明：分离效率随流率的变化存在着高效分离区和基本分离区，高效分离区的脱水率达70%以上，而在较高的处理量下，聚结分离床仍具有一定的基本分离效果。

OBJECTIVE: To construct a prokaryotic expression plasmid of the main catalytic domain of dipeptidyl peptidase of murine fibroblast activation protein αand obtain the target fusion protein in order to provide basis for production of antibodies and anti-tumor vaccine targeting FAPα.

目的：构建小鼠成纤维细胞激活蛋白α二肽基肽酶催化核心序列的高效原核表达载体，进行该序列的原核表达、纯化和复性，为下一步高效抗体制作和以FAPα为靶标的抗肿瘤疫苗研发提供实验基础。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了