高效

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

This paper studied the comprehensive evaluation of beta-cypermethrin pesticides used in aquiculture.

本研究对高效氯氰菊酯杀虫剂在水产养殖上的安全应用进行综合性评价。

Heat transfer analysis of the ground and aquifer involves complex problems in such a case.

单井回灌地源热泵系统是一种高效、环保的新技术，但其涉及的地下水流动和传热问题相当复杂。

Objective To establish a HPLC-ELSD method for the determination of arabinose.

目的建立高效液相色谱-蒸发光散射检测法测定阿拉伯糖含量的方法。

Glucose,formic acid and levulinic acid were not detected in the liquors of acid hydrolysis and the concentration of xylose and arabinose was high,while the acetic acid was low.

目前，国内外用高效液相色谱法测定糖类化合物已有许多报道[4-10]，但同时分析单糖及脂肪酸的报道仅在Solang等[3]的研究中略有叙述。

E. block-pulse functions , a arbitrary piecewise constant functions which are orthogonal and unconcerned are introduced to analyze and synthesize fluid PWM system to solve some related problems of PWM system.

第一章首先通过对流体脉冲调制及相关技术的研究发展现状综述，阐明了寻求高效的分析与设计手段以推动流体脉冲调制系统研究、发展和应用的必要性和重要意义。

Usually, distribution networks operate in arborescence mode, so in the simulation of fault analysis the impact of faulty switch element on system reliability is apportioned to failure consequences of adjacent sections that connect to switch, and then an equivalent analytical model considering switch fault is proposed. Thus, not having to conduct the reliability analysis of switch element singly, the impact of switch element fault on system reliability can be calculated. Applying the proposed algorithm in RBTS-BDS6 system, RBTS-BUS4 system and actual medium-voltage distribution network to conduct reliability assessment, the results show that the proposed algorithm is superior in speed and possesses efficiency and engineering practicality.

配电网常具有树状运行特点，在故障解析模拟时，将开关元件故障对系统可靠性的影响分摊到连接开关的相邻分块的故障后果中，进而提出计及开关故障的可靠性等值解析模型，不需单独进行开关元件故障的可靠性分析即可计算其对系统可靠性的影响，应用该算法对RBTS-BUS6和RBTS-BUS4系统及实际中压配电网进行了可靠性评估，结果表明该算法有较明显的速度优势，具有高效性和工程实用性。

An efficient parallel algorithm, PMAR, which is based on share nothing architechure in multiple computers, is given to settle the problem of mining association rules in a distributed database or a very large database, An incremental updating algorithm for mining association rules, EUIA, which is based on algorithm LIDBSO, is proposed.

提出了一个基于多台计算机间无共享体系结构的高效并行采掘关联规则的算法PMAR，用于解决在分布式数据库中或在特大数据库中的关联规则采掘问题。

The modeling process of fillet and architrave is complex and time-consuming.

针对工程中复杂而完美的倒角、线角建模常常需要巨大的时间代价，本文提出了一种基于特征与参数化相结合的高效建模技术。

The modeling process of fillet and architrave is complex and time-consuming.In this paper,a new efficient modeling algorithm is proposed following the architectural principle of polyline.

针对工程中复杂而完美的倒角、线角建模常常需要巨大的时间代价，本文提出了一种基于特征与参数化相结合的高效建模技术。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。