高低

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

In this study, the brill pos tagger and the claws7 pos tagger have been used to assign pos tags to the same set of transcribed spoken data produced by a high-proficiency group and a low-proficiency group of efl learners. the pos tags have then been manually examined, and the success rates of the taggerscompared.

本研究采用brill和claws7两种词性赋码器分别为高低分组学习者口语语料进行自动赋码，统计赋码准确率，并比较两种赋码器对学习者口语特征的处理能力，目的在于考察基于规则的词性赋码器和基于概率的词性赋码器对学习者口语语料进行自动词性赋码的适用度，以期为中介语口语的句法特点研究提供依据。

Observe the level of foot tarsal surface, and the shoes are too tight before the face of the gap or too loose.

观察脚跗面的高低，与鞋前脸的空档是否太紧或过松。

After joining the European Union in May 2004, Poland got an infusion of cash to modernize its choked roads, antiquated railways and tatty public buildings.

在2004年加入欧盟后，波兰获得了资金援助，以将其高低不平的公路、陈旧落后的铁路以及年久失修的公共建筑重整一新。

The medical equipments are important material base of the medical treatment,scientific research and teaching in the hospital.

医疗设备是医院医疗、科研、教学的重要物质基础。拥有现代化医疗设备的数量多少和质量高低，是衡量一所医院技术水平的重要标志之一。

Again, In addition to the iron on the turntable scale temperature in the laundry there will also be clearly marked.

这除了在熨斗上会有温度高低的转盘刻度外，在衣服洗标上也会有清楚的标示。

Apply tetrachloroethylene to handle carbon black by hot machine method,because of the active double bond of Tetrachloroethylene and the space steric effect of chloride ion.

炭黑在橡胶中的分散状态和分散难易程度，是关系到混炼胶质量高低的关键因素。

An undulating landscape is the new connection between the new and old living quarters of a villa in St.

这处高低起伏的景观是别墅里新旧生活区的新连接点。

Xinhua's 2007 story said He was 13 when she won the championship on uneven bars at the National City Games in central China's Wuhan city last year.

新华社在2007年的报道中称，何可欣13岁，她在去年在武汉举行的全国运动会上获得了高低杆的冠军。

The Chinese last week defeated the Americans to win the team competition, and He won gold in the uneven bars over Nastia Liukin of the U.S.

上周，中国打败美国赢得团体赛冠军，而何可欣则在高低杆上击败了美国队的柳金。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。