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The protein had the same antigenicity as IgG, and the change of the protein had the same tendency as SHP-2, which was higher in canceration groups than in uncanceration groups and control groups in thymus cells, but there weren't significant change in bone marrow cells;⑧ The change law of the expression of SHP-1 and SHP-2 during γ-ray induced malignant transformation in BEAS-2B cell is that the expression of SHP-1 was increased synchronistically with malignant transformation of cell, and the expression of SHP-2 didn't have significant correlation with malignant transformation of cell.

癌变组胸腺细胞中SHP-2 mRNA编码PTPase催化区的700～1096bp间可能出现甲基化；⑦癌变组的胸腺细胞中存在一相对分子质量约为55×10〓的蛋白质，它与IgG具有相同的抗原性，其表达量的变化趋势与SHP-2的蛋白表达变化趋势基本一致，即在胸腺细胞中明显高于对照组及未癌变组，但在骨髓细胞中对照组高于癌变组；⑧SHP-1、SHP-2在γ射线诱发的BEAS-2B细胞恶性转化过程的变化规律：SHP-1蛋白表达量的升高与细胞的恶性转化在时间上基本一致，而SHP-2蛋白表达量与恶性转化无明显关系。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

The studies was proceeded on the the supercritical CO2 extraction technique of rotenone from Tephrosia vogelii. The extraction influences of technology conditions were systematically groped, The research results were listed as follows:1 The analysis method applied in this paper has been proved to be suitable to technology research of the studies on the SC-CO: extraction technique of rotenone from Tephrosia vogelii. 3 indexes, extraction ratio of rotenone. extraction ratio of total compounds and rotenone content percent in total compounds weight, were carried out with HPLC quantitatively determination combined with the sample weight loss after extraction. With these 3 indexes. The extraction influences of technology conditions were evaluated by synthesis. Then the best technology conditions were ascertained for different extraction aims.2 The pretreatment of rough material should be needed before extraction. Comminuting the material to 0.28mm size is most fit to SC-CO: extraction.3 Firstly static extracting 20 min then turn to dynamic doing is the better extraction mode than other ones.4 The orthogonal experiment was conduct with 3 factors and 5 levers.

本论文通过对非洲山毛豆SC-CO_2萃取中影响萃取效果的工艺条件的研究，初步得出如下结果：(1)本论文中所采用的萃取效果评价方法适宜于非洲山毛豆中鱼藤酮的超临界流体萃取技术研究，该方法具体为：HPLC定量分析非洲山毛豆SC-CO_2萃取物中的鱼藤酮含量，再结合萃取前后处理样品的重量损失，得出鱼藤酮萃取率、萃取所得粗物质萃取率和粗物质中鱼藤酮含量三个参数，以此三个指标综合评价萃取条件对萃取效果的影响；(2)通过对原料粒度对萃取效果影响的研究，结果表明SC-CO_2萃取非洲山毛豆复叶中的鱼藤酮，原料粉碎成30目粒度叶粉时萃取效果较好；(3)通过对萃取方式与萃取效果之间关系的研究，结果表明SC-CO_2萃取非洲山毛豆中鱼藤酮应采用先静态后动态的萃取方式，静态萃取时间为20min；(4)通过温度、压力和CO_2流量三因素五水平的正交试验，结果表明温度是最主要的影响因素，压力因素对鱼藤酮萃取率的影响程度高于CO_2流量，而对于粗物质萃取率和粗物质中鱼藤酮含量CO_2流量因素高于压力。

The result of different thawing temperatures indicated that the spermatozoa thawn in 38℃ water showed a better survival rate than those thawn in 50℃ water; the duration of preservation on above 30% survival rate were 4 and 1h, respectively.

结果显示：3%甘油冷冻解冻后精液的活率、活力(38.54%、52.24%)显著(P.05)高于2%甘油组(15.63%、28.22%)，但3%甘油组顶体完整率(11.55%)显著(P.05)低于2%甘油组(21.71%)。38℃水浴解冻后的精子在39℃培养箱中4h内活率高于0.3，而50℃水浴解冻后1h后的活率低于0.3。

Results showed that the activity of soil enzymes was obviously different.Among the different vegetation types,the activity of soil enzymes under Tilia mandshurica forests was the highest except peroxidase,whereas that under shrub-grass was the lowest.The activity of soil urease and alkaline phosphate under shrub was higher than that under the mixed forests.Activity of soil catalase and phosphate under Larix principis-rupprechtii forests was higher than that under Pinus tabulaeformis.

结果表明：不同植被类型的土壤酶活性有显著的区别，其中除过氧化物酶外，糠椴林的土壤酶活性均属最高水平，而灌草丛所有酶活性都属最低水平；灌木丛中脲酶和碱性磷酸酶活性显著高于杂木林；华北落叶松林的磷酸酶和过氧化氢酶活性均高于油松林。

Both of the contents were rich in toona sinensis bud and declined rapidly in cold storage .

结果表明香椿具有较高的抗氧自由基成分，SOD酶活力高于对照栽培蔬菜30%以上，维生素C含量高于对照蔬菜40%以上，但SOD和维生素C在冷藏过程中均有明显的破坏。

To 89. 905 (accounting for 1. 448%-7. 886% in 1, 140bp). The number of transitionsal pairs were far more transversion and higher probability at 1〓, 2〓 than 3〓.

转换发生的数目显著高于颠换，无论是转换还是颠换，在密码子的第三位碱基发生的概率均远高于第一和第二位密码子。

Dactylon, M. triquetra. At tillering, species richness, Simpon index and Shannon-Wiener index are higher while Pielou index is lower in SF than in CK. From booting to ripening, weed species richness, Simpon index and Shannon-Wiener index are lower while Pielou index is significantly higher in SF than in CK.

研究还表明，规模稻鸭生态种养水稻分蘖期物种丰富度、Simpon指数、Shannon-Wiener指数略高于水稻单一种植，Pielou指数低于水稻单一种植；孕穗期到成熟期物种丰富度、Simpon指数、Shannon-Wiener指数低于水稻单一种植，Pielou指数显著高于水稻单一种植。

CD1a and CD83 molecules could also be expressed on the surface of CD3+ T lymphocytes and CD19+ B lymphocytes. The expression levels of CD1a and CD83 in activated lymphocytes were higher than those in the unactivated lymphocytes, and higher in B lymphocytes than in T lymphocytes.

结果该细胞表达特征性分子CD1a、CD40、CD80、CD83、CD86， CD1a 、CD83在CD3+T、CD19+B淋巴细胞上也表达，在激活的淋巴细胞上表达水平高于未激活的淋巴细胞，而且B淋巴细胞上的表达水平高于T淋巴细胞。

The expression of MMP-3 mRNA of RA was higher than that of controls,and the expression of MMP-3 mRNA of RA in active stage was higher than that of RA in unactive stage, and there were no difference in different genotypes.

RA患者外周血单核细胞MMP-3的mRNA表达水平高于健康对照人群，有显著性差异，活动期RA患者外周血单核细胞MMP-3的mRNA表达水平高于非活动期的患者，有显著性差异。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。