髓鞘化

Antibodies including mouse antihuman neurofilament,rabbit anti-human myelin basic protein,rabbit anti-human Al,rabbit anti-human IgM,mouse anti-human IgG,mouse anti-human CD31,mouseanti-human GFAP,mouse anti-human Ki-67,rabbit anti-human serotonin(5-HT),rabbit anti-human SYN,and mouse anti-human c-fos protein were used for immu-nohistochemistry with S-P method.The colorant was brown yellow DAB.

免疫组化染色用S-P法，一抗分别为鼠抗人神经丝蛋白、兔抗人髓鞘碱性蛋白、兔抗人Al、兔抗人IgM、鼠抗人IgG、鼠抗人CD31、鼠抗人GFAP、鼠抗人Ki-67、兔抗人血清素（5-HT）、兔抗人SYN及鼠抗人C-fos蛋白，显色剂为棕黄色DAB。

Results: electronic microscope showed that in prednisone group heterochromatin increased in some cells, entoblast was not obvious in some cells, fat drops were small and few, mitochondria vacuole were common, and myelin figure was found in smooth endoplamic reticulum. the ultrastructure in yc group was almost normal and megamitochondrion formation was common. a large amount of fat drops were found in cytoplasm in gw group and little improvement was found compared with that in prednisone group.

结果： 电镜结果显示：激素组个别细胞异染色质增多，核仁不明显，脂滴显著变小变少，线粒体空泡化常见，有滑面内质网髓鞘样结构形成；养阴组电镜结构基本正常，其特征是细胞内有较多的巨大线粒体形成；桂附组较激素组改善不明显，其特征是胞质内可见大量脂滴。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

SCI can cause hematomyel ia, dropsy, necrosis, capsular space, gummosis and so on, which can lead to neuron deprivation, demyelination of neurite. Clinical symptoms mainly manifest funct ion disorder of movement, sense, reflectance, and musculus sphincter below injury plan.

SCI后出现脊髓出血、水肿、坏死、囊腔形成、退变胶质化等一系列病理改变，以致神经元丧失、轴突脱髓鞘，临床表现为受损平面以下的运动、感觉、反射及括约肌功能的障碍。

Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein histochemical staining and nerve growth factor receptor immunohistochemical staining.

6周后组织切片，行 HE染色、嗜银染色以及髓鞘碱性蛋白和抗神经生长因子受体抗体免疫组化检查。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Extracerebral tumor do not contain neuron and can not be detected NAA in 1HMRS, so we can distinguish glioma and meningioma.

正常新生儿Cho为最高峰，NAA低于Cho，1岁后随着髓鞘化的逐渐完成，NAA高于Cho，正常新生儿看不到Lac。

The stage of neuron development during which many axons become myelinated by glial cells .

神经胶质细胞造成轴突髓鞘化这段期间的神经元发展阶段。

The signal intensity characteristic of MRI associated with brain myelinization is helpful to differentiate the normal and abnormal brain maturation.Also,T1WI is much more useful in the detection of immature myelin and in monitoring of normal brain development in the first 6 months of life.On the other hand,T2WI is more useful after 6 months of life.

不同月龄段不同解剖部位正常脑髓鞘化具有各自MRI信号特征，有利于评判是否脑发育正常和患脑白质病，T1WI评估未成熟髓鞘较好，T2WI确定髓鞘是否成熟较好。

After embeding routingly,serial 10μm sections were prepared for modified trichromic staining with improve-ments to manifest axons and myelin sheaths simultaneously,immunohistochemistryand in situ hybridization.

组化染色用新的改良三色法同时显示神经轴突和神经髓鞘。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。